| Mesoamerican Barrier Reef System coral buoyant weights from the Sapodilla Caye, Belize from UNC starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC.
| Aalborg Mass Flow Controller |
| Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC.
| Aalborg Mass Flow Controller |
| Porites coral calcification responses to declining Ωar in a CO2 manipulation experiment in Palau versus the calcification responses observed in ten other studies of massive Porites corals | Each coral cup was also bubbled with either compressed air or mixed compressed air and CO2 gas controlled by pairs of mass flow controllers (Aalborg Instruments).
| Aalborg Instruments mass flow controllers GFCS-010554 and GFCS-011067 |
| Calcification rates of Porites corals collected from a naturally high-Ωar reef and a naturally low-Ωar reef in Palau incubated at three experimental Ωar conditions | Each coral cup was also bubbled with either compressed air or mixed compressed air and CO2 gas controlled by pairs of mass flow controllers (Aalborg Instruments).
| Aalborg Instruments mass flow controllers GFCS-010554 and GFCS-011067 |
| Calcification rates of communities and sediment in a flume from Richard B Gump Research Station - Moorea LTER, French Polynesia from 2013 (MCR LTER project, OA_Corals project) | Aquacontroller, Neptune systems, USA
| pH-stat |
| Carbonate chemistry parameters in flume study during the day from Richard B Gump Research Station - Moorea LTER, French Polynesia from 2013 (MCR LTER project, OA_Corals project) | Aquacontroller, Neptune systems, USA
| pH-stat |
| Results from OA/feeding experiment: carbonate chemistry and coral skeletal weight, symbiont density, and total tissue lipid content of samples collected from northwestern Bermuda patch reefs; 2010 | A pair of Aalborg mass flow controllers maintained the CO2 concentration of the enriched treatment.
| Mass Flow Controller |
| Coral post-settlement physiology at high pCO2: respiration, protein content, seawater properties and carbonate chemistry, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project) | Qubit Systems Inc.©, Ontario, Canada
| MFC |
| Carbonate data in experimental treatments of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Carbon and nitrogen content of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Experimental results: Emiliania huxleyi growth rates under different pCO2 levels from Bellingham, WA from 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Experimental results: Emiliania huxleyi growth rates under different pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| SEM based morphology of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project) | Sierra Instruments
| MFC |
| Experimental seawater chemical properties for larval mussel shell study from SIO, UCSD from 2012-2013 (OA_Proxies project) | Omega® mass flow controllers (N2: FMA 5418 0–5 SLM; O2: FMA 5411 0–2 SLM; and CO2: FMA 5402 0–10 sccm). See Bockman (2013) for further details.
| MFC |
| Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project) | Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
| Aalborg Mass Flow Controller |
| Experimental results: calcification by primary coral polyps under high bicarbonate and low pH from 2007-2008 (OA Nutrition and Coral Calcification project) | Tthe aquarium seawater pCO2 and DIC levels were set by bubbling with air from a compressor room separate from the lab, and with air+CO2 mixtures produced with pairs of mass flow controllers.
| MFC |
| The density (mg CaCO3/cm^3) of the skeleton of Clathromorphum nereostratum, when assessed as function of increasing seawater temperature and pCO2 concentration | | Darhor manual flow controllers |
| Experimental study to estimate per capita sea urchin (Strongylocentrotus polyacanthus) grazing rates on the alga Clathromorphum nereostratum as a function of seawater temperature and pCO2 concentration | | Darhor manual flow controllers |
| Calcification and linear extension rates for Oculina arbuscula corals grown under different pCO2 levels; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project) | The experimental air-CO2 gases were formulated using Aalborg mass flow controllers. See more information from Aalborg.
| MFC |
| Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project) | Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
| Aalborg Mass Flow Controller |
| Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) | Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
| Aalborg Mass Flow Controller |
| Pleurochrysis carterae growth cycle culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) | Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
| Aalborg Mass Flow Controller |
| Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project) | Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
| Aalborg Mass Flow Controller |
| Experimental results of Plocamium cartilagineum growth and biomass as a function of pCO2 and temperature (Seaweed OA Resilience project) | Used to mix gasses: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.
| Qubit Systems Mass Flow Controllers (MFC) |
| Ramped pyrolysis oxidation (RPO) carbon isotope data from DOC in water samples collected during R/V Western Flyer Benthic-Pelagic Coupling expedition to Station M in the eastern Pacific Ocean in April 2018 | The RPO system consists of a flow system (with mass flow controllers and infrared gas analyzer), a furnace system, and a vacuum separations system.
| mass flow controller |
| Ramped pyrolysis oxidation (RPO) thermogram data of DOC from water samples collected during R/V Western Flyer Benthic-Pelagic Coupling expedition to Station M in the eastern Pacific Ocean in April 2018 | The RPO system consists of a flow system (with mass flow controllers and infrared gas analyzer), a furnace system, and a vacuum separations system.
| mass flow controller |
| Results from a study of physiological responses of Ulva lactuca to ocean acidification and nutrient enrichment | Mass flow controlled (MFC) gas mixing system (Qubit Systems, Ontario, Canada)
| Mass flow controlled (MFC) gas mixing system |
| Symbiodinium cell density, chlorophyll, respiration, photosynthetic yield in Seriatopora coral, Taiwan, 2010 (MCR LTER project, Climate_Coral_Larvae project) | A350 Gas Concentration Controller (Qubit) solenoid-controlled
| MFC |
| Seriatopora bleaching expt: Photosynthesis:irradiance (P/I) curves, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project) | A350 Gas Concentration Controller (Qubit); solenoid-controlled
| MFC |
| Seriatopora bleaching expt: temperature, salinity, light in tanks, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project) | A350 Gas Concentration Controller (Qubit); solenoid-controlled
| MFC |
| Seriatopora bleaching expt: water pH and carbonate chemistry, Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project) | A350 Gas Concentration Controller (Qubit); solenoid-controlled
| MFC |
| Data on growth rates, and physiological parameters of Ulva australis under ocean acidification (OA) and eutrophication, from July 2015 (Seaweed OA Resilience project) | Three pairs of MFC's were used to achieve the appropriate mix of N2 and CO2 gas.
| Omega Engineering, models FMA5418A and FMA545C |
| Time-series of estimating pH in culture tanks of Ulva australis under ocean acidification (OA) and eutrophication (Seaweed OA Resilience project) | Three pairs of MFC's were used to achieve the appropriate mix of N2 and CO2 gas.
| Omega Engineering, models FMA5418A and FMA545C |
| Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project) | Settings: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.
| Qubit Systems Mass Flow Controllers (MFC) |
| Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project) | Settings: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.
| Qubit Systems Mass Flow Controllers (MFC) |
| Weekly coral calcification rates in the CO2 manipulation experiment conducted on Porites corals from Palau | Each coral cup was also bubbled with either compressed air or mixed compressed air and CO2 gas controlled by pairs of mass flow controllers (Aalborg Instruments).
| Aalborg Instruments mass flow controllers GFCS-010554 and GFCS-011067 |
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