A free-flushing water sample bottle comprising a cylinder (polycarbonate, acrylic or PVC) with a stopper at each end. The bottle is closed by means of a messenger from the surface releasing the tension on a latex band and thus pulling the two stoppers firmly into place. A thermometer can be mounted inside the bottle. One or more bottles can be lowered on a line to allow sampling at a single or multiple depth levels. Van Dorn samplers are suitable for for physical (temperature), chemical and biological sampling in shallow to very deep water. Bottles are typically lowered vertically through the water column although a horizontal version is available for sampling near the seabed or at thermoclines or chemoclines. Because of the lack of metal parts the bottles are suitable for trace metal sampling, although the blue polyurethane seal used in the Alpha version may leach mercury. The Beta version uses white ASA plastic seals that do not leach mercury but are less durable.