©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.The FACSCalibur flow cytometer is an autonomous benchtop flow cytometer designed for routine cell analysis, assay development, verification and identification of cellular populations. It is equipped with a blue (488 nm) air-cooled argon laser and a red (635 nm) diode laser. For each particle (cell), five optical parameters can be recorded from the 488 nm laser beam excitation: two light scatter signals, namely forward and right angle, and three fluorescences corresponding to emissions in green (530/30 nm BP), orange (585/42 nm BP) and red (670 nm LP) wavelength ranges. A far red fluorescence (661/16 nm BP) induced by the red diode can also be recorded. Data are analysed using BD Biosciences CellQuest software. Optional features include a cell sorting option, allowing users to identify and isolate a population of interest and a HTS option (High-throughput (HT) or Standard (STD) mode), where sample volumes range from 2-10 microlitres in HT mode and 2-200 microlitres in STD mode. An optional BD FACS Loader tube-lifter can be used to verify tube position and rack identification. The instrument has a capture rate of 300 cells per second, supports 40 (12 x 75 mm) tubes per rack, and has an operating temperature ranging from 16-29 degC.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Time series observations: hydrography, nutrients, and microbial, phytoplankton and zooplankton communities from the Skidaway River Estuary on the southeastern Atlantic coast of Georgia from 1986-2011 (SRiMP project) | BD FACSCalibur flow cytometer Since 2012, a variety of flow cytometric instruments are utilized to quantify picoplankton and larger microplankton including diatoms and dinoflagellates in our SRiMP program. Picoplankton analysis is performed by Dr. Liz Mann using a FacsCalibur (BD Biosciences, San Jose, CA, USA) in house. Additionally, we employ a state of the art CytoSense benchtop flow cytometer equipped with an imaging system and wide (1.5 mm) flow-cell uniquely capable of rapid analysis of live cells including large and chain-forming species (up to 4 mm long) such as diatoms and dinoflagellates (CytoBouy b.v., Woerden, the Netherlands, http://www.cytobuoy.com) performed in house by Dr. Jens Nejstgaard. | BD FACSCalibur flow cytometer |