©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.The Hydrothermal Organic Geochemistry (HOG) sampler is designed to collect large volume (2–9 L) fluid samples with minimal introduction of organic or microbial contamination, and to be powered and deployed in real time from a submersible. Additional design constraints include utilizing materials appropriate for sampling fluids with elevated temperatures, fitting the sampler into the space available on the submersible, and minimizing the time needed to remove samples and prepare the sampler for re-deployment between dives.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Lost City hydrothermal fluids sequence data from R/V Atlantis AT42-01 in the Lost City hydrothermal field from September to October 2018 (Lost City Limits to Life project) | On the seafloor, venting fluids were slowly pumped through 0.2 μm Millipore Sterivex cartridge filters or into acid-washed Kynar bags with the HOG sampler. Samples intended for RNA extractions were collected into 2 L Kynar bags containing 67 mL of a stop solution (97.5% 200 proof ethanol, 2.5% Trizol LS). Fluid temperatures were monitored in real-time during sampling with a probe embedded into the sampler intake. | HOG (hydrothermal organic geochemistry) sampler |
| Metagenome and metatranscriptome sequences from deep-sea hydrothermal vent microbial communities collected on cruises AT42-22, TN405, and NA108 from May 2019 to Jun 2022 | All samples were collected via ROVs, where an intake valve was positioned at the location of active diffuse flow ( | ROV Jason, HOG sampler |