Project: Oligotrophic phytoplankton community response to changes in N substrates and the resulting impact on genetic, taxonomic and functional diversity

Acronym/Short Name:PhytoNsubResponse
Project Duration:2013-01 - 2015-12
Geolocation:North Pacific Subtropical Gyre at Station ALOHA, and a transect from San Diego, CA to Hawaii

Description

(Extracted from NSF award abstract)

Marine phytoplankton are a diverse group of Prokaryotic and Eukaryotic unicellular organisms that account for approximately 50% of global carbon fixation. Nitrogen (N) is an essential element for microbial growth, but concentrations of bioavailable nitrogen in vast regions of subtropical ocean gyres are extremely low (submicromolar to nanomolar concentrations), and generally limit phytoplankton growth. Phytoplankton taxa differ in their genetic capabilities to take up and assimilate nutrients, and thus competition for different chemical forms of N (NH4+, NO3- and urea) and supply of these N-containing compounds are important controls on phytoplankton growth, productivity, and ultimately ecosystem function. The form and supply of N to phytoplankton have already been altered by anthropogenic activities, and with increasing environmental perturbations the effects will accelerate. To date however, there is limited information on how the N forms and fluxes impact the marine phytoplankton community composition and primary production. Similarly, determining the mechanisms of the response are crucial to assessing how ocean ecosystem function will respond to global climate change.

This project seeks to determine how taxonomic, genetic and functional dimensions of phytoplankton diversity are linked with community-level responses to the availability of different N substrates (NH4+, NO3-, and urea) in one of Earth's largest aquatic habitats, the North Pacific Subtropical Gyre. The project will characterize phytoplankton community composition change and gene expression, photosynthetic performance, carbon fixation, and single-cell level N and C uptake in different taxa within the phytoplankton assemblage in response to different N compounds. The research project is unique in investigating community-to-single-cell level function and species (strain)-specific gene expression patterns using state-of-the-art methods including fast repetition rate fluorometry, nanoscale secondary ion mass spectrometry and a comprehensive marine microbial community microarray. The results will provide predictive understanding of how changes in the availability of key nitrogen pools (N) may impact phytoplankton dynamics and function in the ocean.

References:

Karl, D. M., Bjorkman, K. M., Dore, J. E., Fujieki, L., Hebel, D. V., Houlihan, T., Letelier, R. M., Tupas, L. M. 2001. Ecological nitrogen-to-phosphorus stoichiometry at station ALOHA. Deep-Sea Research II. 48:1529 - 1566.

Karl, D. M., Letelier, R., Tupas, L., Dore, J., Christian, J. & Hebel, D. 1997. The role of nitrogen fixation in biogeochemical cycling in the subtropical North Pacific Ocean. Nature. 388:533-538.

McCarthy, J., Taylor, W. R., Taft, J. 1997.  Nitrogenous nutrition of the plankton in the Chesapeake Bay. Limnology and Oceanography. 35:822 - 829.

Letelier, R., Karl, D. M. 1996.  Role of Trichodesmium spp. in the productivity of the subtropical North Pacific Ocean. Marine Ecology Progress Series. 133:263 - 273.

Lipschultz, F. 1995.  Nitrogen-specific uptake rates of marine phytoplankton isolated from natura populations of particles by flow cytometry. Marine Ecology Progress Series. 123:245-258.


DatasetLatest Version DateCurrent State
Phytoplankton MicroTOOLs microarray-based metatranscriptomes collected from a nutrient amendment experiment conducted during R/V New Horizon Cruise NH1417 in the California Current System from 2014-08-23 to 2014-08-262019-07-18Final no updates expected
Niskin bottle sample data from R/V New Horizon NEMO cruise NH1417 in the Eastern Pacific between San Diego and Hawaii from August to September 2014 (Phyto response to N substrates project)2018-03-09Preliminary and in progress
Size fractionated and group-specific rates of 14C-primary production by Prochlorococcus, Synechococcus, and photosynthetic picoeukaryotes from samples collected during Hawaii Ocean Time-series (HOT) program cruises 230-252 between 2012 and 20132017-08-25Final no updates expected
18S rRNA gene sequence from the euphotic zone at Station ALOHA from near-monthly samples collected during Hawaii Ocean Time-series (HOT) program cruises 230-252 between 2011 and 2013 (PhytoNsubResponse project)2017-08-25Final no updates expected
CTD profile downcasts from R/V New Horizon NEMO cruise NH1417 in the Eastern Pacific between San Diego and Hawaii from August to September 2014 (Phyto response to N substrates project)2017-03-23Preliminary and in progress
Cruise tracks from R/V New Horizon NH1417 in the Eastern Pacific between San Diego and Hawaii in 2014 (Phyto response to N substrates project)2016-01-11Final no updates expected
Size-fractioned primary production (14C-bicarbonate assimilation rates) from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Preliminary and in progress
Phytoplankton pigments from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Preliminary and in progress
Photosynthetic picoplankton abundances from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Preliminary and in progress
Measurements of inorganic nutrients (nitrate+nitrite, phosphate, silicate, and ammonium) from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Preliminary and in progress

People

Lead Principal Investigator: Jonathan P. Zehr
University of California-Santa Cruz (UCSC)

Principal Investigator: Kevin R. Arrigo
Stanford University

Principal Investigator: Matthew J. Church
University of Hawaiʻi at Mānoa (SOEST)

Co-Principal Investigator: Dr Zbigniew Kolber
University of California-Santa Cruz (UCSC)


Programs

Dimensions of Biodiversity [Dimensions of Biodiversity]