Description from NSF award abstract:
Dust and mineral aerosols are a significant source of micro and macronutrients to oligotrophic ocean surface waters. Evidence is growing that heterotrophic microbes may play key roles in processing deposited minerals and nutrients. Yet it is not known which components of dust stimulate the heterotrophic bacteria, which cellular mechanisms are responsible for the utilization of those components and how the activity of these bacteria affect the availability and utilization of dust-derived minerals and nutrients by marine autotrophs. Knowledge of these factors is key to understanding how dust deposition impacts carbon cycles and for predicting the response of tropical oceans to future changes in the frequency and intensity of dust deposition events. The objective of this project is to examine the specific effects of aeolian dust on heterotrophic microbes in a tropical marine system under controlled conditions. The central hypothesis is that in oligotrophic tropical systems numerically minor opportunistic bacteria are the first responders to influx of dust constituents and respond primarily by rapidly accessing soluble trace metals and limiting nutrients that are deposited with Saharan dust. The project will focus on two specific aims: 1) Quantify changes in community structure, composition and transcriptional activity among marine microbial populations upon exposure to dust, and 2) Identify key components in Saharan dust aerosols that stimulate or repress growth and/or activity in Vibrio, a model opportunistic marine heterotrophic group. The study will use a series of controlled experiments designed to identify and quantify heterotrophic microbial response to dust deposition events using both natural communities and model bacteria (Vibrio) through metagenomics, transcriptomics and atmospheric and marine biogeochemical techniques. This innovative approach will identify the most critical (reactive) components leached from dust aerosols on the microbial community as well as elucidate potential mechanisms of response.
There is great interest in the biological response to dust aerosols given its potentially large influence on biogeochemical cycling, but there has been relatively little work that has addressed the mechanisms of response (especially among the heterotrophic microbial fraction) or identified the relative importance of specific constituents of dust aerosols. A detailed framework for microbial response (focusing on opportunistic heterotrophs) will facilitate efforts to link autotrophic and heterotrophic processing. This contribution is significant because it will provide one of the first end-to-end (chemistry to physiology to ecology) mechanistic pathways for marine biological response to desert dust aerosols.
Dataset | Latest Version Date | Current State |
---|---|---|
Accession numbers for raw sequences associated with field collections & microcosms, 2015 and 2016 | 2018-06-19 | Final no updates expected |
Aerosol trace element concentrations in the Florida Keys National Marine Sanctuary from 2014-2015 (Vibrio-dust deposition project) | 2017-08-02 | Under revision |
Sea surface microlayer trace element concentrations from Florida Keys National Marine Sanctuary from 2014-2015 (Vibrio-dust deposition project) | 2017-08-02 | Final no updates expected |
Nutrients, microbiology, trace metals, and environmental conditions from seeded microcosm experiments | 2016-11-09 | Final with updates expected |
Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016 | 2016-11-03 | Final with updates expected |
Lead Principal Investigator: Erin K. Lipp
University of Georgia (UGA)
Principal Investigator: William M. Landing
Florida State University (FSU - EOAS)
Principal Investigator: Michael Wetz
Texas A&M, Corpus Christi (TAMU-CC)
Co-Principal Investigator: Elizabeth Ottesen
University of Georgia (UGA)
Contact: Erin K. Lipp
University of Georgia (UGA)
Data Management Plan received by BCO-DMO on 23 March 2015. (223.55 KB)
03/23/2015