©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.Description from C-DEBI website:
The aim of this proposal is to gain a better understanding of what subsets of proteins are actually being expressed during neutrophilic, microbial iron (Fe)-oxidation. The recently isolated Mariprofundus ferrooxydans, strain PV-1, will be used as a marine model organism to investigate proteomic differences under different Fe substrates: aqueous Fe2+ and solid Fe0. Two-dimensional gel electrophoresis (2D-GE) and shotgun proteomic methods (LC-MS/MS) will be employed to obtain results from the cultures grown under different conditions. The research being proposed would constitute the foundation for the development of diagnostic tools for the accordance, distribution, and activity level of Fe-oxidation, a globally important biogeochemical process at and below the ocean floor.
This project was funded by a C-DEBI Graduate Student Fellowship.
| Dataset | Latest Version Date | Current State |
|---|---|---|
| Raw LC-MS/MS data, with list of identified peptides, and DNA sequences from a proteomic profile of Mariprofundus ferrooxydans | 2016-01-28 | Final no updates expected |
| Raw LC-MS/MS data with list of identified peptides in xml format from a proteomic analysis performed on protein extracts of M. ferrooxydans | 2016-01-27 | Final no updates expected |
Principal Investigator: Roman A. Barco
Bigelow Laboratory for Ocean Sciences
Co-Principal Investigator: Katrina Edwards
University of Southern California (USC)
Center for Dark Energy Biosphere Investigations [C-DEBI]