Award: OCE-1850719

Award Title: Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions
Funding Source: NSF Division of Ocean Sciences (NSF OCE)
Program Manager: Daniel Thornhill

Outcomes Report

The physiology, biochemistry and biogeography of nitrogen-fixing cyanobacteria and unicellular picocyanobacteria are strongly influenced by temperature, subjecting them to intense selective pressure as the modern ocean steadily warms up. These groups have likewise been rigorously selected under chronic iron (Fe) scarcity, and the availability of this crucial micronutrient is also changing with a shifting climate. This project examined short-term acclimation and long-term evolutionary responses of Fe-stressed marine cyanobacteria to a warmer environment. In addition, this project assessed the long-term adaptive responses to Fe limitation and warming interactions in major cyanobacterial groups. Through these studies, information was gained that contributes to a mechanistic and predictive understanding of adaptation to Fe and warming co-stressors in a rapidly changing future ocean environment for some of the most important photoautotrophic functional groups in the ocean. This project also applied a new method for localizing the use of metals within cells, known as metalloproteomics, to characterize how metal micronutrition is influenced by environmental change. Novel molecules that store iron within marine cyanobacteria were identified. This project supported the development of the Ocean Protein Portal module for classroom based research that can provide access to authentic research experiences to students that may not otherwise have access to scientific research. Research training was provided to graduate students, postdocs and technicians in the course of this grant. Last Modified: 03/22/2024 Submitted by: MakASaito
DatasetLatest Version DateCurrent State
Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 20182020-01-10Final no updates expected
Metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 20182020-01-10Final no updates expected
Trichodesmium sample provenance from samples collected in North Atlantic surface waters, station BATS, and station ALOHA between 2000 and 20182020-01-10Final no updates expected
Net tow metaproteome of Trichodesmium species mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes in units of normalized protein spectral counts from samples collected in the Atlantic and Pacific Ocean between 2000 and 20182020-04-30Final no updates expected
Net tow metaproteoome of Trichodesmium species mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes in units of normalized peptide spectral counts from samples collected in the Atlantic and Pacific Ocean between 2000 and 20182020-04-30Final no updates expected
FASTA file of sequences in Trichodesmium field metaproteomes mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes from samples collected in the Atlantic and Pacific Ocean between 2000 and 20182020-01-14Final no updates expected
Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 20162020-04-21Final no updates expected
Growth rates of the polar diatom Chaetoceros RS19 under various +Zn and +Co conditions from September 2019 (MM Saito project)2021-08-31Final no updates expected
Metal quotas (ratios of Metal:P) of the polar diatom Chaetoceros sp. RS19 in +Zn and +Co incubation studies from January 2020 (MM Saito project)2021-08-30Final no updates expected
Metal uptake rates of the polar diatom Chaetoceros RS19 in +Zn and +Co incubation studies from January 2020 (MM Saito project)2021-08-30Final no updates expected

Award Home Page

NSF Research Results Report


People

Principal Investigator: Mak A. Saito (Woods Hole Oceanographic Institution)