A combination of pyrosequencing and QPCR approaches were used to examine rRNA:rDNA ratios, BrdU incorporating cells, and transcript types and amounts in the metatranscriptome of Sargasso Sea surface water. The investigators also used Micro-FISH to examine incorporation of thymidine, leucine, and PO4. Samples were collected in 2010 during the spring phytoplankton bloom when heterotrophic bacterial production is lowest and during the peak of bacterial production in summer.
Campbell, B., Heidelberg, J., Kirchman, D. L. (2014) Paired metagenomic and metatranscriptomic data from R/V Atlantic Explorer AE1006, AE1024, Bermuda Atlantic Time Series Station (BATS), Sargasso Sea, Mar and Aug 2010 (Active bacteria in surface waters project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2014-04-23) Version Date 2014-04-23 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/512782 [access date]
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