©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.DNA extracts from the vicinity of Station ALOHA (22.75 N, 158.0 W) just north of Hawaii.
CMORE RNA and DNA Archive
Goal: The goal of this effort is to provide a time series collection of planktonic microbial DNA and RNA depth profiles from the HOT station, for CMORE research. DNA and RNA samples will be available for researchers to conduct and coordinate taxonomic and functional gene studies, for example using PCR and RT-PCR methods. Where possible, metagenomic datasets will be generated by pyrosequencing for selected profiles, and posted for downloading the blast searching on the MIT-CMORE server (http://genesis2.mit.edu/).
Activity: Collect microbial cell fraction (1.6um prefiltered, >0.22um) from HOT hydrocasts, and extract nucleic acids, for RNA and DNA for downstream analyses by CMORE investigators. Collection depths are 25m, 45m, 75m, 125m, 200m, 500m, 770m and 1000m. Extracted DNA samples will be available for distribution.
DeLong, E. (2015) DNA extracts from the vicinity of Station ALOHA (22.75 N, 158.0 W) just north of Hawaii from 2007-2015 (C-MORE project, HOT project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2015-03-31 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/517403 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.