Dataset: DNA metagenomic library statistics from HOT cruises from 2007-2009 (C-MORE project, HOT project)

Final no updates expectedVersion 01 April 2015 (2015-04-01)Dataset Type:Unknown

Principal Investigator: Edward DeLong (Massachusetts Institute of Technology)

Data Manager: Jasmine Nahorniak (Oregon State University)

BCO-DMO Data Manager: Stephen R. Gegg (Woods Hole Oceanographic Institution)


Program: Ocean Carbon and Biogeochemistry (OCB)

Program: U.S. Joint Global Ocean Flux Study (U.S. JGOFS)

Program: Ocean Time-series Sites (Ocean Time-series)

Project: Center for Microbial Oceanography: Research and Education (C-MORE)

Project: [Current] Hawaii Ocean Time-series (HOT): 2023-2028; [Previous] Hawaii Ocean Time-series (HOT): Sustaining ocean ecosystem and climate observations in the North Pacific Subtropical Gyre (HOT)

HOT DNA metagenomic library statistics

Location: The vicinity of Station ALOHA (22.75 N, 158.0 W) just north of Hawaii.

CMORE RNA and DNA Archive

Goal: The goal of this effort is to provide a time series collection of planktonic microbial DNA and RNA depth profiles from the HOT station, for CMORE research. DNA and RNA samples will be available for researchers to conduct and coordinate taxonomic and functional gene studies, for example using PCR and RT-PCR methods. Sequencing files are available as an NCBI BioProject (PRJNA16339)

Activity: Collect microbial cell fraction (1.6um prefiltered, >0.22um) from HOT hydrocasts, and extract nucleic acids, for RNA and DNA for downstream analyses by CMORE investigators. Collection depths are 25m, 45m, 75m, 125m, 200m, 500m, 770m and 1000m. Extracted DNA samples will be available for distribution.


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