Water was pumped to the surface using a trace metal clean diaphragm pump and acid cleaned teflon tubing and dispensed into trace metal clean (TMC) 50 L carboys. Sampling occurred between 18:00 and 19:00 in open air, with wind coming strictly from over open water, NNE to NE. The carboys were protected from light with dark plastic bags and returned to the Crary Laboratory at McMurdo Station via helicopter within one hour of sampling.
Chl a was measured using 90% acetone extracts and the non-acidified fluorometric method, employing a Turner Designs fluorometer and Whatman GF/F filters.
Nitrate (NO3-), nitrite (NO2-), phosphate (PO43-) and silicate (SiO4) concentrations were measured in duplicate on a Lachat QuikChem 8500 autoanalyzer. Ammonium (NH4+) concentrations were measured in triplicate using the phenol-hypochlorite method.
Bronk, D. A., Sipler, R. E., Hutchins, D. A., Allen, A. E. (2015) Nutrients and chlorophyll from sea water samples collected in the Ross Sea from 2013-2015 (Ross Sea Microb Ecophys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-12-14) Version Date 2015-12-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/628244 [access date]
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