Dataset: Transcript counts per liter from samples collected during the SIMCO 1 & 2 (July & Oct 2014) incubation experiments on the linkage between DOM changes and microbial transcription patterns at Sapelo Island, GA.

Final no updates expectedVersion (2016-10-26)Dataset Type:Other Field Results

Principal Investigator: Mary Ann Moran (University of Georgia)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: High Resolution Linkages Between DOC Turnover and Bacterioplankton in a Coastal Ocean (SIMCO)

Incubation experiments examining concurrent changes in DOC composition and microbial transcription patterns were carried out in July 2014 (SIMCO1) and October 2014 (SIMCO2) at Sapelo Island, GA. This dataset contains the number of transcripts per liter of seawater for each taxon. Cells were collected by filtration at the time of initial surface water collection (Time 0) and after a 24 hr incubation (Time 24) for both high and low tide water collections on each of the two sample dates. Filters were processed for RNA extraction and mRNA-enriched material was sequenced on an Illumina HiSeq2500 run to produce single-end 250 bp reads.


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