©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
Glycine betaine, an inhibitor of bacterial DMSP uptake, was added at 5 micromolar concentrations to triplicate samples; triplicate controls had no addition. Subsamples were removed at the initial time point and at 2 hr intervals up to 6 hrs. Samples were analyzed for remaining DMSPd and the difference between glycine betaine and control incubations was calculated to estimate DMSP consumption rate.
For methodology details, see:
Varaljay, V., et al. Single-taxon field measurements of bacterial gene regulation controlling DMSP fate. The ISME Journal (2015), 1-10. doi:10.1038/ismej.2015.23
Rellinger, A., et al. Occurrence and turnover of DMSP and DMS in deep waters of the Ross Sea, Antarctica. Deep-Sea Research I 56 (2009) 686–702. doi:10.1016/j.dsr.2008.12.010
Li, C., et al, Assessment of DMSP turnover reveals a non-bioavailable pool of dissolved DMSP in coastal waters of the Gulf of Mexico. Environ. Chem. (2015) http://dx.doi.org/10.1071/EN15052
Moran, M., Birch, J. M., Kiene, R. P. (2016) Dimethylsulfoniopropionate (DMSP) concentrations and turnover during a deployment of the Environmental Sample Processor in Fall, 2014 in Monterey Bay, CA. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version ) Version Date 2016-10-25 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/662421 [access date]
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©2020 Biological and Chemical Oceanography Data Management Office.