Dataset: Cell counts of symbionts in Muricea atlantica, M. elongata, and Plexaurella dichotoma across the 2015 Bleaching event (May 2015 to August 2017) at Long Key in the Florida Keys

Final no updates expectedDOI: 10.26008/1912/bco-dmo.718585.3Version 3 (2017-11-29)Dataset Type:Other Field Results

Principal Investigator: Mary Alice Coffroth (State University of New York at Buffalo)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Project: RAPID: Variations in symbiont diversity in octocoral across seasons and a predicted bleaching event (Octocoral symbiont diversity)

Abstract

Cell counts of symbionts in Muricea atlantica, M. elongata, and Plexaurella dichotoma across the 2015 Bleaching event (May 2015 to August 2017) at Long Key in the Florida Keys.

Cite Dataset

Location:  
Two patch reefs in the vicinity of Long Key in the Florida Keys (CMF-N24.44.704, W 80 46.793 and SC2- N24 49.478 W80 41.187).

Sampling and Analytical Methodology:  
Except for M. atlantica, which was not sampled in May 2015, tagged colonies were sampled in September 2015 (during bleaching), November 2015, March 2016, May 2016, September 2016, November 2016, March 2017 and August 2017 (after bleaching).  Symbiont density within coral tissues was determined for each collection. Coral tissue was preserved in 1.0 ml of 5% formalin.  For each sample, the length and diameter of each sample were measured to the nearest 0.1 mm using vernier calipers, and the tissue then homogenized in distilled water. The density of symbiont cells in the homogenate was counted at least 4 consecutive times, using standard hemocytometer procedures and converted to cells per tissue volume using the formula for a cylinder to calculate tissue volume.

Taxonomic identifiers (Genus species, LSID):
Muricea atlantica, urn:lsid:marinespecies.org:taxname:287554
Muricea elongata, urn:lsid:marinespecies.org:taxname:287559
Plexaurella dichotoma, urn:lsid:marinespecies.org:taxname:290812

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Related Publications

Results
Coffroth, M.A., Buccella L., Eaton K. M., Franklin H., Gooding A. T., Lasker, H. R. (submitted) Octocoral thermotolerance may signal resilience to major bleaching events: roles of symbiont and host. Submitted to Global Change Biology
Methods
ANDRAS, J. P., KIRK, N. L., & DREW HARVELL, C. (2011). Range-wide population genetic structure of Symbiodinium associated with the Caribbean Sea fan coral, Gorgonia ventalina. Molecular Ecology, 20(12), 2525–2542. https://doi.org/10.1111/j.1365-294x.2011.05115.x
Methods
Coffroth, M. A., Lasker, H. R., Diamond, M. E., Bruenn, J. A., & Bermingham, E. (1992). DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species. Marine Biology, 114(2), 317–325. doi:10.1007/bf00349534
Methods
Pettay, D. T., & Lajeunesse, T. C. (2007). Microsatellites from clade B Symbiodinium spp. specialized for Caribbean corals in the genus Madracis. Molecular Ecology Notes, 7(6), 1271–1274. doi:10.1111/j.1471-8286.2007.01852.x
Methods
Santos, S. R., Gutierrez-Rodriguez, C., & Coffroth, M. A. (2003). Phylogenetic identification of symbiotic dinoflagellates via length heteroplasmy in Domain V of chloroplast Large Subunit (cp23S)-Ribosomal DNA Sequences. Marine Biotechnology, 5(2), 130–140. doi:10.1007/s10126-002-0076-z

Coffroth, M. A. (2023) Cell counts of symbionts in Muricea atlantica, M. elongata, and Plexaurella dichotoma across the 2015 Bleaching event (May 2015 to August 2017) at Long Key in the Florida Keys. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 3) Version Date 2017-11-29 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.718585.3 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


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Funded by the U.S. National Science Foundation