Dataset: Colony forming units (CFUs) of water samples from Mariana and Kermadec trenches grown in 3 media from R/V Falkor FK141109, FK141215, and R/V Thompson TN309, 2014 (Mariana Perspectives project)

Final no updates expectedVersion (2017-12-14)Dataset Type:experimental

Principal Investigator: Douglas Bartlett (University of California-San Diego Scripps)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Patterns of Microbial Community Structure Within and Between Hadal Environments (Mariana Perspectives)

This dataset includes counts of colony forming units (CFUs) from seawater samples from the Mariana and Kermadec trenches from April, November, and December 2014.

NOTE: sample RG10 in this dataset is equivalent to RG09 in the event log, due to at-sea recording error. RG10-2 is equivalent to RG10 in the event log.


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Methods

Fuhrman JA, Comeau DE, Hagstrom A, Chan AM. Extraction from natural plankton microorganisms of DNA suitable for molecular biological studies. Appl Environ Microbiol. 1988;54: 1426-1429.
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Rusch, D. B., Halpern, A. L., Sutton, G., Heidelberg, K. B., Williamson, S., Yooseph, S., … Venter, J. C. (2007). The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific. PLoS Biology, 5(3), e77. doi:10.1371/journal.pbio.0050077
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Tarn, J., Peoples, L. M., Hardy, K., Cameron, J., & Bartlett, D. H. (2016). Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench. Frontiers in Microbiology, 7. doi:10.3389/fmicb.2016.00665
Methods

Yayanos, A. Aristides (2001). Deep-sea piezophilic bacteria. Marine Microbiology, 615–637. doi:10.1016/s0580-9517(01)30065-x