Dataset: Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradiance

Release Date:2020-09-30Final no updates expectedDOI: 10.26008/1912/bco-dmo.746398.1Version 1 (2018-09-18)Dataset Type:experimental

Principal Investigator: Edward Laws (Louisiana State University College of the Coast and Environment)

Co-Principal Investigator: Uta Passow (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)


Abstract

Thalassiosira pseudonanana were grown in nitrate limited culture at five temperatures, 400 umol photons m–2 s­–1, and 1000 ppm CO2 and irradiance levels of 50 and 300 umol photons m-2 s-1. Growth rates, photosynthetic rates, respiration rates, C:N ratio, C:Chlorophyll-a ratio, maximum quantum yield are reported.

Thalassiosira pseudonanana were grown in nitrate limited culture at five temperatures, 400 umol photons m–2 s­–1, and 1000 ppm CO2 and irradiance levels of 50 and 300 umol photons m-2 s-1. Growth rates, photosynthetic rates, respiration rates, C:N ratio, C:Chlorophyll-a ratio, maximum quantum yield are reported.


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Methods

Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. ISBN: 1-897176-07-4. URL: https://www.nodc.noaa.gov/ocads/oceans/Handbook_2007.html
Methods

Millero, F. J., Zhang, J.-Z., Fiol, S., Sotolongo, S., Roy, R. N., Lee, K., & Mane, S. (1993). The use of buffers to measure the pH of seawater. Marine Chemistry, 44(2-4), 143–152. doi:10.1016/0304-4203(93)90199-x
Methods

Zeebe, R. E., & Wolf-Gladrow, D. (2001). CO2 in seawater: equilibrium, kinetics, isotopes (No. 65). Gulf Professional Publishing.