| File(s) | Type | Description | Action |
|---|---|---|---|
Fe_label_Ecoli_T4_T5.csv (126.12 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 757485 | |
| Supplemental File(s) | Type | Description | Action |
Table2.PNG (33.46 KB) | Portable Network Graphics (.png) | Table 2: Analytical blank values for 56Fe and 57Fe after rhodium correction as measured on ELEMENT XR ICP-MS. “Expt” refers to experimental and analytical dataset; e.g. Expt 2.1 is experiment 2, first analytical dataset, while Expt 2.2 is experiment 2, second analytical dataset. The number of blank measurements used to calculate the average and standard deviation for each round is “n”. | |
Table1.PNG (72.58 KB) | Portable Network Graphics (.png) | Table 1: M9 minimal media composition from Kutter and Sulakvelidze (2004). A 1 L stock of 20X M9 Salts and 0.5 L of all stock solutions were weighed separately in acid-cleaned Nalgene fluorinated HDPE bottles and brought to desired concentrations using Milli-Q water (18.2 MΩ cm). A 1 L volume of liquid culture was prepared by adding all sterile stock solution components to 912.5 mL of Milli-Q water in an acid-cleaned HDPE bottle on a balance. *FeSO4 added as 57FeSO4 spike separately after all other components combined. Completed M9 minimal media was 0.02 µm filtered prior to use. | |
Figure3.PNG (151.65 KB) | Plain Text | Figure 3: Flowchart of the Tracing 57Fe to E. coli phages T4 & T5 Experimental method. First, cell cultures were grown and aliquoted into triplicate samples of each treatment: T4 infected (A, B, C), T5 infected (M, N, O), Bacterial Control (D, E, F), and Blank Control (G, H, I). Cell cultures were rinsed to remove excess 57Fe, then lysed by phage T4 infection, phage T5 infection, or chloroform for the bacterial lysis control. The lysates are then purified for the phage by centrifugation, filtration, sucrose ultracentrifugation, and dialysis. | |
Figure2.PNG (129.69 KB) | Portable Network Graphics (.png) | Figure 2: Schematic of E. coli cell preparation | |
Figure1.PNG (34.65 KB) | Portable Network Graphics (.png) | Growth curve for E. coli ZK126 measured on a spectrophotometer. Mid-logarithmic growth (indicated by shaded area) was an OD600= 0.200-0.500. |
Files
Filename: Fe_label_Ecoli_T4_T5.csv (126.12 KB)
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 757485
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 757485
Supplemental Files
Filename: Table2.PNG (33.46 KB)
Type: Portable Network Graphics (.png)
Description: Table 2: Analytical blank values for 56Fe and 57Fe after rhodium correction as measured on ELEMENT XR ICP-MS. “Expt” refers to experimental and analytical dataset; e.g. Expt 2.1 is experiment 2, first analytical dataset, while Expt 2.2 is experiment 2, second analytical dataset. The number of blank measurements used to calculate the average and standard deviation for each round is “n”.
Type: Portable Network Graphics (.png)
Description: Table 2: Analytical blank values for 56Fe and 57Fe after rhodium correction as measured on ELEMENT XR ICP-MS. “Expt” refers to experimental and analytical dataset; e.g. Expt 2.1 is experiment 2, first analytical dataset, while Expt 2.2 is experiment 2, second analytical dataset. The number of blank measurements used to calculate the average and standard deviation for each round is “n”.
Filename: Table1.PNG (72.58 KB)
Type: Portable Network Graphics (.png)
Description: Table 1: M9 minimal media composition from Kutter and Sulakvelidze (2004). A 1 L stock of 20X M9 Salts and 0.5 L of all stock solutions were weighed separately in acid-cleaned Nalgene fluorinated HDPE bottles and brought to desired concentrations using Milli-Q water (18.2 MΩ cm). A 1 L volume of liquid culture was prepared by adding all sterile stock solution components to 912.5 mL of Milli-Q water in an acid-cleaned HDPE bottle on a balance. *FeSO4 added as 57FeSO4 spike separately after all other components combined. Completed M9 minimal media was 0.02 µm filtered prior to use.
Type: Portable Network Graphics (.png)
Description: Table 1: M9 minimal media composition from Kutter and Sulakvelidze (2004). A 1 L stock of 20X M9 Salts and 0.5 L of all stock solutions were weighed separately in acid-cleaned Nalgene fluorinated HDPE bottles and brought to desired concentrations using Milli-Q water (18.2 MΩ cm). A 1 L volume of liquid culture was prepared by adding all sterile stock solution components to 912.5 mL of Milli-Q water in an acid-cleaned HDPE bottle on a balance. *FeSO4 added as 57FeSO4 spike separately after all other components combined. Completed M9 minimal media was 0.02 µm filtered prior to use.
Filename: Figure3.PNG (151.65 KB)
Type: Plain Text
Description: Figure 3: Flowchart of the Tracing 57Fe to E. coli phages T4 & T5 Experimental method. First, cell cultures were grown and aliquoted into triplicate samples of each treatment: T4 infected (A, B, C), T5 infected (M, N, O), Bacterial Control (D, E, F), and Blank Control (G, H, I). Cell cultures were rinsed to remove excess 57Fe, then lysed by phage T4 infection, phage T5 infection, or chloroform for the bacterial lysis control. The lysates are then purified for the phage by centrifugation, filtration, sucrose ultracentrifugation, and dialysis.
Type: Plain Text
Description: Figure 3: Flowchart of the Tracing 57Fe to E. coli phages T4 & T5 Experimental method. First, cell cultures were grown and aliquoted into triplicate samples of each treatment: T4 infected (A, B, C), T5 infected (M, N, O), Bacterial Control (D, E, F), and Blank Control (G, H, I). Cell cultures were rinsed to remove excess 57Fe, then lysed by phage T4 infection, phage T5 infection, or chloroform for the bacterial lysis control. The lysates are then purified for the phage by centrifugation, filtration, sucrose ultracentrifugation, and dialysis.
Filename: Figure2.PNG (129.69 KB)
Type: Portable Network Graphics (.png)
Description: Figure 2: Schematic of E. coli cell preparation
Type: Portable Network Graphics (.png)
Description: Figure 2: Schematic of E. coli cell preparation
Filename: Figure1.PNG (34.65 KB)
Type: Portable Network Graphics (.png)
Description: Growth curve for E. coli ZK126 measured on a spectrophotometer. Mid-logarithmic growth (indicated by shaded area) was an OD600= 0.200-0.500.
Type: Portable Network Graphics (.png)
Description: Growth curve for E. coli ZK126 measured on a spectrophotometer. Mid-logarithmic growth (indicated by shaded area) was an OD600= 0.200-0.500.