Dataset: Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures

Final no updates expectedDOI: 10.26008/1912/bco-dmo.780155.1Version 1 (2019-10-28)Dataset Type:experimental

Principal Investigator: Elena Litchman (Michigan State University)

Co-Principal Investigator, Contact: Daniel R. O'Donnell (Michigan State University)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Dimensions of Biodiversity (Dimensions of Biodiversity)

Project: Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients (Phytoplankton Community Responses)


Abstract

Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures.

Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures.

These data were published in O'Donnell and Litchman (2019) Evol. Appl. and can also be found in Supporting Information file eva12798-sup-0001-Supinfo.docx, Figures 1-5 of the main text, and on Dryad: https://datadryad.org/stash/dataset/doi:10.5061/dryad.pn04m84.


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Results

O’Donnell, D. R., Du, Z., & Litchman, E. (2019). Experimental evolution of phytoplankton fatty acid thermal reaction norms. Evolutionary Applications, 12(6), 1201–1211. doi:10.1111/eva.12798
Methods

Boyle, N. R., Page, M. D., Liu, B., Blaby, I. K., Casero, D., Kropat, J., … Merchant, S. S. (2012). Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation inChlamydomonas. Journal of Biological Chemistry, 287(19), 15811–15825. doi:10.1074/jbc.m111.334052
Methods

O’Donnell, D. R., Hamman, C. R., Johnson, E. C., Kremer, C. T., Klausmeier, C. A., & Litchman, E. (2018). Rapid thermal adaptation in a marine diatom reveals constraints and trade-offs. Global Change Biology, 24(10), 4554–4565. doi:10.1111/gcb.14360
Methods

Wang, Z., & Benning, C. (2011). <em>Arabidopsis thaliana</em> Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC). Journal of Visualized Experiments, (49). doi:10.3791/2518