Dataset: Metabarcoding samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017.

Final no updates expectedDOI: 10.26008/1912/bco-dmo.824599.1Version 1 (2020-09-11)Dataset Type:Cruise Results

Principal Investigator: Lisa Campbell (Texas A&M University)

Co-Principal Investigator: Darren W. Henrichs (Texas A&M University)

Contact: Chetan Gaonkar (Texas A&M University)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: RAPID: Hurricane Impact on Phytoplankton Community Dynamics and Metabolic Response (HRR)


Abstract

Metabarcode samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017.

Metabarcode samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017.


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Results

Gaonkar, C. C., Piredda, R., Sarno, D., Zingone, A., Montresor, M., & Kooistra, W. H. C. F. (2020). Species detection and delineation in the marine planktonic diatoms Chaetoceros and Bacteriastrum through metabarcoding: making biological sense of haplotype diversity. Environmental Microbiology, 22(5), 1917–1929. doi:10.1111/1462-2920.14984
General

Guillou, L., Bachar, D., Audic, S., Bass, D., Berney, C., Bittner, L., ... & Christen, R. (2012). The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote small sub-unit rRNA sequences with curated taxonomy. Nucleic acids research, 41(D1), D597-D604.
Methods

Bradley, I. M., Pinto, A. J., & Guest, J. S. (2016). Design and Evaluation of Illumina MiSeq-Compatible, 18S rRNA Gene-Specific Primers for Improved Characterization of Mixed Phototrophic Communities. Applied and Environmental Microbiology, 82(19), 5878–5891. doi:10.1128/aem.01630-16
Methods

Edgar, R. C., Haas, B. J., Clemente, J. C., Quince, C., & Knight, R. (2011). UCHIME improves sensitivity and speed of chimera detection. Bioinformatics, 27(16), 2194–2200. doi:10.1093/bioinformatics/btr381
Methods

Kozich, J. J., Westcott, S. L., Baxter, N. T., Highlander, S. K., & Schloss, P. D. (2013). Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology, 79(17), 5112–5120. doi:10.1128/aem.01043-13