| File(s) | Type | Description | Action |
|---|---|---|---|
metabarcode.csv (1.80 MB) | Comma Separated Values (.csv) | Primary data file for dataset ID 824599 | |
| Supplemental File(s) | Type | Description | Action |
station_locations.png (461.29 KB) | Portable Network Graphics (.png) | Sampling locations and identification of the station abbreviations | |
locations_metatrans.csv (445.00 B) | Comma Separated Values (.csv) | Table with locations for each of the station-leg collection sites. | |
HRR_Karenia_phylogeny.pdf (1.22 MB) | Portable Document Format (.pdf) | Fig 2. Karenia phylogeny: Selection of appropriate metabarcoding marker for species detection and delineation. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Karenia species. OTUs based on (a) V4-region and (b) V8-V9 regions of 18S rDNA gene yield different results. Numbers on the internodes indicate the bootstrap values if ≥50 (1000 replicates). Only those reference sequences which had full length 18S rDNA sequences were selected, and Karenia reference sequences are indicated with a colored dot. Only OTUs those with read abundance more than 100 were selected. | |
HRR_Chaetocerotacean_phylogeny.pdf (73.72 KB) | Portable Document Format (.pdf) | Fig. 1. Chaetocerotacean diversity: Assessing the Chaetocerotacean diversity using the V4-hypervaiable region of 18S rDNA using HTS-metabarcoding. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Chaetocerotacean species from the sample collected in the Gulf of Mexico, Texas coast. A total of 206 validated Chaetocerotacean metabarcode haplotypes along with 150 taxonomically validated Chaetocerotacean reference sequences and 26 outgroup sequences were used to generate the phylogenetic trees to assess species diversity. The OTU ID consists of the dataset name, unique identification number and number of reads at the end. Numbers on the internodes indicate bootstrap values if ≥50 (1000 replicates). |
Files
Filename: metabarcode.csv (1.80 MB)
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 824599
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 824599
Supplemental Files
Filename: station_locations.png (461.29 KB)
Type: Portable Network Graphics (.png)
Description: Sampling locations and identification of the station abbreviations
Type: Portable Network Graphics (.png)
Description: Sampling locations and identification of the station abbreviations
Filename: locations_metatrans.csv (445.00 B)
Type: Comma Separated Values (.csv)
Description: Table with locations for each of the station-leg collection sites.
Type: Comma Separated Values (.csv)
Description: Table with locations for each of the station-leg collection sites.
Filename: HRR_Karenia_phylogeny.pdf (1.22 MB)
Type: Portable Document Format (.pdf)
Description: Fig 2. Karenia phylogeny: Selection of appropriate metabarcoding marker for species detection and delineation. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Karenia species. OTUs based on (a) V4-region and (b) V8-V9 regions of 18S rDNA gene yield different results. Numbers on the internodes indicate the bootstrap values if ≥50 (1000 replicates). Only those reference sequences which had full length 18S rDNA sequences were selected, and Karenia reference sequences are indicated with a colored dot. Only OTUs those with read abundance more than 100 were selected.
Type: Portable Document Format (.pdf)
Description: Fig 2. Karenia phylogeny: Selection of appropriate metabarcoding marker for species detection and delineation. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Karenia species. OTUs based on (a) V4-region and (b) V8-V9 regions of 18S rDNA gene yield different results. Numbers on the internodes indicate the bootstrap values if ≥50 (1000 replicates). Only those reference sequences which had full length 18S rDNA sequences were selected, and Karenia reference sequences are indicated with a colored dot. Only OTUs those with read abundance more than 100 were selected.
Filename: HRR_Chaetocerotacean_phylogeny.pdf (73.72 KB)
Type: Portable Document Format (.pdf)
Description: Fig. 1. Chaetocerotacean diversity: Assessing the Chaetocerotacean diversity using the V4-hypervaiable region of 18S rDNA using HTS-metabarcoding. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Chaetocerotacean species from the sample collected in the Gulf of Mexico, Texas coast. A total of 206 validated Chaetocerotacean metabarcode haplotypes along with 150 taxonomically validated Chaetocerotacean reference sequences and 26 outgroup sequences were used to generate the phylogenetic trees to assess species diversity. The OTU ID consists of the dataset name, unique identification number and number of reads at the end. Numbers on the internodes indicate bootstrap values if ≥50 (1000 replicates).
Type: Portable Document Format (.pdf)
Description: Fig. 1. Chaetocerotacean diversity: Assessing the Chaetocerotacean diversity using the V4-hypervaiable region of 18S rDNA using HTS-metabarcoding. Maximum likelihood tree generated using raxmlGUI v2.0 (Edler et al. 2019) for the Chaetocerotacean species from the sample collected in the Gulf of Mexico, Texas coast. A total of 206 validated Chaetocerotacean metabarcode haplotypes along with 150 taxonomically validated Chaetocerotacean reference sequences and 26 outgroup sequences were used to generate the phylogenetic trees to assess species diversity. The OTU ID consists of the dataset name, unique identification number and number of reads at the end. Numbers on the internodes indicate bootstrap values if ≥50 (1000 replicates).