Dataset: Sample and treatment information from NMR assays of metabolite experiments on Thalassiosira pseudonanna 1335 inoculated with three bacterial strains

Release Date:2020-11-04Final no updates expectedDOI: 10.26008/1912/bco-dmo.826583.1Version 1 (2020-10-14)Dataset Type:experimental

Principal Investigator: Arthur Edison (University of Georgia)

Contact: Nicole R. Holderman (University of Georgia)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Marine Microbiology Initiative (MMI)

Project: Metabolic Currencies of the Ocean Carbon Cycle (Metabolic Currencies)

Project: Assessing the Movement of Carbon Currencies Between Marine Microbes (Carbon Currencies Microbes)


Abstract

This dataset includes information about samples and treatments analyzed with NMR. Bacteria Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152 were collected 48 hours after individual inoculation into a diatom Thalassiosira psuedonana culture and the resulting metabolites were analyzed. Raw data files are located at Metabolights, MetaboLights accession number MTBLS1544.Raw data files are located at Metabolights, MetaboLights accession number MTBLS1544.

The study examined the ability of bacteria to draw down exometabolites from the culture medium as detected by novel approaches using magnetic resonance (NMR) analysis. See related dataset on parallel experiments using mass spectrometry (MS).This dataset includes information about samples and treatments analyzed with NMR. Bacteria Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152 were collected 48 hours after individual inoculation into a diatom Thalassiosira psuedonana culture and resulting metabolites were analysed.

Raw data files are located at Metabolights, MetaboLights accession number MTBLS1544;
https://www.ebi.ac.uk/metabolights/MTBLS1544/files
https://www.ebi.ac.uk/metabolights/MTBLS1544/samples


Related Datasets

IsSupplementTo

Dataset: https://www.ebi.ac.uk/metabolights/MTBLS1544/samples
Frank X Ferrer-Gonzalez, Brittany Widner, Nicole R Holderman, John Glushka, Arthur S Edison, Elizabeth B Kujawinski, Mary Ann Moran (2020) MTBLS1544: Resource Partitioning of Diatom Metabolites that Support Bacterial Heterotrophy in the Ocean (NMR assay). In: MetaboLights [Internet], EMBL-EBI, Wellcome Genome Campus, Hinxton, Cambridgeshire, CB10 1SD, UK. Available from: https://www.ebi.ac.uk/metabolights/MTBLS1544/descriptors, MetaboLights study: MTBLS1544.

Related Publications

Results

Ferrer-González, F. X., Widner, B., Holderman, N. R., Glushka, J., Edison, A. S., Kujawinski, E. B., & Moran, M. A. (2020). Resource partitioning of phytoplankton metabolites that support bacterial heterotrophy. The ISME Journal. doi:10.1038/s41396-020-00811-y