Dataset: Series 2A-2: Multiple stressor experiments on T. pseudonana (CCMP1014) – photophysiology

Final no updates expectedDOI: 10.26008/1912/bco-dmo.829695.1Version 1 (2020-11-18)Dataset Type:experimental

Principal Investigator: Uta Passow (University of California-Santa Barbara)

Co-Principal Investigator: Edward Laws (Louisiana State University)

Scientist, Contact: Julia Sweet (University of California-Santa Barbara)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Effects of multiple stressors on Marine Phytoplankton (Stressors on Marine Phytoplankton)

Abstract

These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. Experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. Six temperatures (13.5°C, 20°C, 25°C, 29°C, 31°C, and 32.5°C), and eight light intensities (25, 50, 80, 115,190, 300, 400 and 600 µmol photons · m-2 · s-1 ) were tested during the cou...

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The raw fluorescence data can be found under the Data Files section as an Excel file and as individual .csv files.

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Results
Sweet, J. A. (2020). A tale of two drivers: Exploring the response of the marine diatom, Thalassiosira pseudonana to changes in temperature and irradiance (Order No. 28092894). Available from ProQuest Dissertations & Theses A&I; ProQuest Dissertations & Theses Global. (2455969153). Sweet, J. A. (2020). A tale of two drivers: exploring the response of the marine diatom, Thalassiosira pseudonana to changes in temperature and irradiance. UC Santa Barbara. ProQuest ID: Sweet_ucsb_0035N_14840. Merritt ID: ark:/13030/m5pw207f. Retrieved from https://escholarship.org/uc/item/28m0d4h6
Methods
Dickson, A. G. (1993). The measurement of sea water pH. Marine Chemistry, 44(2-4), 131–142. doi:10.1016/0304-4203(93)90198-w
Methods
Dickson, A. G., & Millero, F. J. (1987). A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733–1743. doi:10.1016/0198-0149(87)90021-5
Methods
Guillard, R. R. L. (1975). Culture of Phytoplankton for Feeding Marine Invertebrates. Culture of Marine Invertebrate Animals, 29–60. doi:10.1007/978-1-4615-8714-9_3
Methods
Kester, D. R., Duedall, I. W., Connors, D. N., & Pytkowicz, R. M. (1967). Preparation of Artificial Seawater 1. Limnology and Oceanography, 12(1), 176–179. doi:10.4319/lo.1967.12.1.0176

Passow, U., Laws, E., Sweet, J. (2020) Series 2A-2: Multiple stressor experiments on T. pseudonana (CCMP1014) – photophysiology. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-11-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.829695.1 [access date]

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This dataset is licensed under Creative Commons Attribution 4.0.


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