Dataset: Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.839645.1Version 1 (2021-02-04)Dataset Type:Other Field Results

Principal Investigator: Tina Treude (University of California-Los Angeles)

Co-Principal Investigator: Sebastian Krause (University of California-Los Angeles)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)


Project: Deciphering the Cryptic Cycling of Methane in Sediments of a Coastal Wetland (Cryptic CH4 Cycling)


Abstract

Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California. Sediments were collected in June 2018 from the hypersaline pool using large and small pushcores. Porewater was separated from sediment by centrifugation and subsampled for further analysis.

Sediments were collected from the hypersaline pool within the Carpinteria Salt Marsh Reserve using large (20 cm i.d.) and small (2.6 cm i.d.) pushcores. Porewater was separated from sediment by centrifugation (4300 x g for 20 mins) and subsampled for further analysis described below.

Porewater sulfate and chloride concentrations were determined by ion chromatography (Metrohm 761). Porewater salinity was calculated from porewater chloride concentrations, using Knudson’s equation (Knudsen, 1901). Porewater sulfide and iron (II) concentrations were determined spectrophotometrically (Shimadzu UV-Spectrophotometer UV-1800) according to Cline, (1969) and Grasshoff et al., (1999) respectively. Porewater methane concentrations were determined by gas chromatography (Shimadzu GC-2014). Sediment porosity was determined by drying sediments at 75°C for five days and calculated using the difference between wet and dry weights, divided by the volume of sediment.

Sulfate reduction rates were determined by injecting carrier-free 35S-Sulfate into intact whole round cores according to Jørgensen (1978); and incubated for 1 day. Sediment samples were then analyzed according to the cold-chromium distillation (Kallmeyer et al., 2004).

AOM rates were determined by injecting 14C-Methane dissolved in anoxic Milliq into intact whole round cores similar to sulfate reduction rate determinations; and incubated for 1 day. Sediment samples were then analyzed according to Treude et al. (2005) and Joye et al. (2004).


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Results

Krause, S. J. E., & Treude, T. (2021). Deciphering cryptic methane cycling: Coupling of methylotrophic methanogenesis and anaerobic oxidation of methane in hypersaline coastal wetland sediment. Geochimica et Cosmochimica Acta, 302, 160–174. doi:10.1016/j.gca.2021.03.021
Methods

1901. Hydrographical tables according to the measurings of Carl Forch, J. P. Jacobsen, Martin Knudsen and S. P . L. Sjijrensen. G. E. C. Gad, Copenhagen (63 pp.). (Second edition 1931 fotoprinted by Tutein & Koch.)
Methods

Cline, J. D. (1969). Spectrophotometric Determination of Hydrogen Sulfide in Natural Waters. Limnology and Oceanography, 14(3), 454–458. doi:10.4319/lo.1969.14.3.0454
Methods

Grasshoff, K., Kremling, K., & Ehrhardt, M. (Eds.). (1999). Methods of Seawater Analysis. doi:10.1002/9783527613984
Methods

Jorgensen, B. B. (1978). A comparison of methods for the quantification of bacterial sulfate reduction in coastal marine sediments. Geomicrobiology Journal, 1(1), 11–27. doi:10.1080/01490457809377721