File(s) | Type | Description | Action |
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microscopy_cell_counts.csv (5.69 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 848977 | Download |
This dataset represents microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. These data were assessed in Anderson et al. The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community (in prep).
Multivariate mesocosm experiments were conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added).
At the onset and conclusion of the incubation experiments, the microplankton community was identified and quantified to discern treatment effects on community composition. Aliquots from each incubation were fixed in triplicate with 2% acid Lugol’s solution for microscopy, final concentration. Cell enumeration was conducted by performing cell counts ~>10 micrometer (µm) on a 1 milliliter (ml) Sedgewick cell-counting chamber (Structure Probe Inc.) using an Eclipse E800 microscope (Nikon).
These data were assessed as part of “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).
Anderson, S. I., Franze, G., Kling, J. D., Wilburn, P., Kremer, C. T., Menden-Deuer, S., Litchman, E., Hutchins, D. A., Rynearson, T. A. (2021) Microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.848977.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.