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hplc_data-1.csv (30.85 KB) | Comma Separated Values (.csv) | Primary data file for dataset 892576, version 1. | Download |
Benthic microalgal photopigment concentrations were measured on selected dates from 7 June 2018 to 10 August 2021 in nearshore shelf waters off Charleston, SC. The survey area was located in the region of 32° 42’ N, 79° 50’ W and 32° 51’ N, 79° 09’ W. Sediment core samples were analyzed by HPLC. These data were used to map the benthic microalgal biomass and assess inputs of groundwater effects on benthic microalgae. Results may be of interest to others conducting research projects off Charleston...
Show moreSamples for Benthic microalgal photopigment analysis were collected by SCUBA divers from the R/V Trinity in nearshore waters of Charleston, SC using 1 square centimeter x 10 centimeter (cm) plastic core tubes gently pushed into the sediment to minimize disruption of the surface layers. Tubes were capped and carefully removed from the sediment. Ten cores were obtained randomly within a 1 square meter area at select locations for each sample date. The upper 1 centimeter of the core was extruded, frozen at -80 degrees Celsius, and analyzed within 30 days after collection. Sediments were dried and sieved for grain size analysis.
High-performance liquid chromatography (HPLC) was used to determine chemosystematic photosynthetic pigments. Samples were lyophilized for 24 hours at -50 degrees Celsius, placed in 90 percent acetone (1 milliliter), sonicated on ice for 30 seconds, and extracted at -20 degrees Celsius for 18 to 20 hours. Filtered extracts: 0.45 micrometers, 250 microliters were injected into a Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 centimeters, 3 micrometers) and a polymeric (Vydac 201TP54, 0.46 x 25 centimeters, 5 micrometers) reverse-phase C18 column in series. A nonlinear binary gradient of 80 percent methanol: 20 percent 0.50 M ammonium acetate and 80 percent methanol:20 percent acetone was the mobile phase (Pinckney et al. 1996, 2001). Absorption spectra and chromatograms (440 ± 4 nanometers) were acquired using a Shimadzu SPD-M10av photodiode array detector. Pigment peaks were identified by comparison of retention times and absorption spectra with pure carotenal and chlorophyll standards (DHI, Denmark). The synthetic carotenoid β-apo-8'-carotenal (Sigma) was used as an internal standard.
Pinckney, J. L. (2023) Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.892576.1 [access date]
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