Dataset: Nutrient concentrations and stable isotope values for groundwater in nearshore shelf waters off Charleston, SC from June 2018 to August 2019

Final no updates expectedDOI: 10.26008/1912/bco-dmo.892997.1Version 1 (2023-04-03)Dataset Type:Other Field Results

Principal Investigator, Contact: James L. Pinckney (University of South Carolina)

BCO-DMO Data Manager: Taylor Heyl (Woods Hole Oceanographic Institution)


Project: Groundwater sources of "new" N for benthic microalgal production in the South Atlantic bight (SAB BMA)


Abstract

Nutrient concentrations and stable isotope values for groundwater were measured on selected dates from 7 June 2018 to 10 August 2019 in nearshore shelf waters off Charleston, SC. The survey area was located in the region of 32° 42’ N, 79° 50’ W and 32° 51’ N, 79° 09’ W. Sediment core samples were analyzed by HPLC. These data were used to map nutrient concentrations and assess inputs of groundwater effects on benthic microalgae. Results may be of interest to others conducting research projects of...

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Nutrient concentrations and stable isotope values for groundwater were measured on selected dates from 7 June 2018 to 10 August 2019 in nearshore shelf waters off Charleston, SC (32° 42’ N, 79° 50’ W and 32° 51’ N, 79° 09’ W). At each of the groundwater wells in the wellfield, divers collected fluids from the wells to determine well water inorganic nutrient concentrations (nitrate + nitrite, ammonium, orthophosphate) and the δ15N of well water DON, ammonium, and nitrate (when present). Sediment core samples were analyzed by HPLC. These data were used to map nutrient concentrations and assess inputs of groundwater effects on benthic microalgae.

δ15N of Nitrate: Filtered (0.2 micrometer) samples were stored in acid-washed HDPE bottles and frozen (-20 degrees Celsius) until analysis. For any sample with more than 0.5 μM NO3-, the isotopic composition of NO3- was determined by the denitrifier method of Sigman et al. (2001) and Casciotti et al. (2002), with updates according to McIlvin & Casciotti (2011) and Weigand et al. (2016).

δ15N of Ammonium: Filtered (0.2 μm) samples will be stored on dry ice in the field and shipped immediately to the lab for δ15N of ammonium analysis. Samples with more than 3 µM NH4+ and adequate sample volume were analyzed using the hypobromite/azide method of Zhang et al. (2007).


Related Datasets

IsRelatedTo

Dataset: Benthic microalgal photopigment concentrations
Pinckney, J. L. (2023) Benthic microalgal photopigment concentrations from nearshore shelf waters off Charleston, SC from June 2018 to August 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-10 doi:10.26008/1912/bco-dmo.892576.1

Related Publications

Results

Pinckney, J. L., Zaunbrecher, S., Lang, S., Wilson, A., & Knapp, A. (2022). Seasonality of benthic microalgal community abundance in shallow shelf waters. Continental Shelf Research, 244, 104797. https://doi.org/10.1016/j.csr.2022.104797
Methods

Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., & Hilkert, A. (2002). Measurement of the Oxygen Isotopic Composition of Nitrate in Seawater and Freshwater Using the Denitrifier Method. Analytical Chemistry, 74(19), 4905–4912. doi:10.1021/ac020113w
Methods

Kofoid, & Campbell, A. S. (1929). A conspectus of the marine and fresh?water Ciliata belonging to the suborder Tintinnoinea, with descriptions of new species principally from the Agassiz Expedition to the eastern tropical Pacific 1904?1905. Univ. Calif. Publs Zool., 34, 1–403.
Methods

McIlvin, M. R., & Casciotti, K. L. (2011). Technical Updates to the Bacterial Method for Nitrate Isotopic Analyses. Analytical Chemistry, 83(5), 1850–1856. doi:10.1021/ac1028984
Methods

Sigman, D. M., Casciotti, K. L., Andreani, M., Barford, C., Galanter, M., & Böhlke, J. K. (2001). A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater. Analytical Chemistry, 73(17), 4145–4153. doi:10.1021/ac010088e