©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.A Fluorescence (or Epifluorescence) Microscope Image Analysis System uses semi-automated color image analysis to determine cell abundance, dimensions and biovolumes from an Epifluorescence Microscope. An Epifluorescence Microscope (conventional and inverted) includes a camera system that generates enlarged images of prepared samples. The microscope uses excitation ultraviolet light and the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Abundance, biovolume and biomass of heterotrophic dinoflagellates from cruises TT007, TT008, TT011, TT012 in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project | fluorescence microscope image analysis system used to measure Abundance and biovolume | Fluorescence Microscope Image Analysis System |
| Reduced Epifluorescence Microscopy Water Column Samples from R/V Tangaroa TAN1810 in the Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand) from October to November 2018 (Salp Food Web Ecology project) | Slides were calibrated from pixels to microns for both 0.8 µm and 8 µm to determine what length of pixels equates to µm thus only micron area, width and feret length are show in the raw datasheet. Our data also includes conversation ratios in the raw excel sheet. This ratio should be included when determining biomass in units of pg C/mL or abundance in #/mL, along with volume filter, as this ratio incorporates the area of the images taken and gives a value that states the ratio of the cells that were actually counted for 0.8 µm and 8 µm samples. | Epifluorescence microscope: Olympus BX51 microscope with a Olympus DP72 camera and Exfo X-cite Series 120 mercury bulb with a FITC filter for green fluorescence |
| Phenotypic plasticity of the ciliated band of seven species of echinoderm larvae, collected between 2020 and 2022 in the laboratory at California State University, Long Beach. | Olympus BX-51 compound microscope outfitted with a QIClick camera (Teledyne Photometrics) and a motorized z-axis drive, both controlled via MicroManager software to create image stacks of fixed larvae for biometric measurements. | |
| Abundance and biomass of protists from epifluorescence counts and bulk biomass from extracted chl-a from samples from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station in 2011-12 | The organisms were counted using a ZEISS Axioplan Epifluorescence Microscope equipped with a 100x Plan-NEOFLUAR 100x/1.30 oil, objective lens | Fluorescence Microscope Image Analysis System |
| Experimental results from a study of pPeudo-nitzschia multiseries domoic acid production, C-fixation, mean growth, and element composition under varying pCO2 and phosphate levels (PhytoTM_in_HighCO2 project) | Cells were enumerated using an Olympus BX51 epifluorescence microscope at 100-fold magnification. | Fluorescence Microscope Image Analysis System |
| Experimental results from a study of Pseudo-nitzschia multiseries growth rates and cellular domoic acid under varying pCO2 and phosphate levels (PhytoTM_in_HighCO2 project) | Cells were enumerated using an Olympus BX51 epifluorescence microscope at 100-fold magnification. | Fluorescence Microscope Image Analysis System |
| Quantification of ciliated band length per unit protein in early echinoderm larvae (biometirc data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. | Olympus BX-51 compound microscope outfitted with a QIClick camera (Teledyne Photometrics) and a motorized z-axis drive, both controlled via MicroManager software to create image stacks of fixed larvae for biometric measurements. | |
| Surface water and N.digitalis inhalent/exhalent water sample nutrient and bacterioplankton metadata of Looe Key and Wonderland Reef conducted in 10-15 December 2023 | Fluorometer was used to measure fDOM | Horiba Aqualog scanning fluorometer |