Water samples are then transferred to 1 L polyethylene storage bottles or 2 L plastic storage bottles, and stored in a dark place at 4 oC until picked up by personnel on the same day.
Total and dissolved nutrient samples will be held in acid-washed 250 mL polyethylene bottles, and stored in a dark freezer at -20 oC until they are ready to be analyzed.
Sample bottles, 1 or 2 L (Nalgene or equivalent), Plastic wash bottle, 500 mL
LDPE bottles: water samples were collected in acid-washed LDPE bottles for trace metals.
Rubber septum glass vials: water samples were collected in ashed glass bottles for nutrients.
Experiments 3 and 4, respectively, were conducted with 10 μm pre-filtered natural seawater and 10 μm pre-filtered sea water first incubated in 20 L bottles (bottle blooms).
Experiments 3 and 4, respectively, were conducted with 10 μm pre-filtered natural seawater and 10 μm pre-filtered sea water first incubated in 20 L bottles (bottle blooms).