Cyprinodon variegatus offspring growth rate from 5 generation increasing temperature experiments conducted on Atlantic wild caught specimens during 2014. | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Temperature and pCO2 effects on survivability of 25 genotypes of Acropora cervicornis coral at Mote Marine Laboratory in Nov-Dec 2019 | Coral fragments were placed into treatment tanks (raceways)
| treatment tanks |
Aquaria water quality PAR measurements from full factorial study of Acropora cervicornis at Mote Marine Laboratory in Nov-Dec 2019 | Aquaria water quality was measured in tanks used for coral treatment experiments
| treatment tank |
Aquaria water quality pH and dissolved oxygen measurements from full factorial study of Acropora cervicornis at Mote Marine Laboratory in Nov-Dec 2019 | Aquaria water quality was measured in tanks used for coral treatment experiments
| tank |
Aquaria water quality total alkalinity, DIC, and CO2 measurements from full factorial study of Acropora cervicornis at Mote Marine Laboratory in Nov-Dec 2019 | Aquaria water quality was measured in tanks used for coral treatment experiments
| treatment tank |
Pilot study with three unique genotypes of Acropora cervicornis coral to determine survival probability after exposure to temperature treatments at Mote Marine Laboratory in September and October 2019 | Coral fragments were placed into treatment tanks (raceways)
| treatment tanks |
Biodiversity experiments: Effects of diversity in feeding trials, conducted at Bodgea Marine Laboratory, using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers | Pre-weighed fragments of eelgrass were covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank.
| indoor tank |
Mesoamerican Barrier Reef System coral buoyant weights from the Sapodilla Caye, Belize from UNC starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC.
| 38 L glass aquaria |
Mesoamerican Barrier Reef System coral buoyant weights for temperature experiments from Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | In the laboratory experiments, Siderastrea siderea coral specimens from each of the 18 colonies were reared for 95 days (5 August − 8 November 2011) in each of twelve 38 L glass aquaria (18 specimens per tank; 216 specimens in total) filled with artificial seawater formulated at a salinity of 35 with Instant Ocean Sea Salt and deionized water. Four pCO2 partial pressures [324, 477, 604, and 2553) ppm)], established by mixing pure CO2 with compressed air using Aalborg mass flow controllers, were bubbled with microporous ceramic airstones into the triplicate glass aquaria (12 tanks total). Coral specimens from each of the 18 colonies were reared in each of the 12 replicate tanks. The pCO2 experiments were maintained at an average temperature of 28 ºC.
| 38 L glass aquaria |
Carbon and nitrogen data from kelp determined during seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species | | |
Results from OA/feeding experiment: carbonate chemistry and coral skeletal weight, symbiont density, and total tissue lipid content of samples collected from northwestern Bermuda patch reefs; 2010 | | Aquarium |
Water samples from CTD casts and vehicle-mounted bottles from the R/V Atlantis, R/V Ronald Brown, R/V Falkor, & E/V Nautilus in the Gulf of Mexico & Florida from 2010 to 2014 (Lophelia OA project) | Experiments conducted using aquaria
| Aquarium |
Major ion and carbonate chemistry of water in which oyster larvae were reared from Whiskey Creek Shellfish Hatchery in Netarts Bay, OR, USA from 2009-2011 | | Tank |
Cyprinodon variegatus offspring growth rate from coloration discovery experiments conducted on specimens wild caught from the Atlantic in 2014 | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Compensatory growth and TGP data from experiments conducted on juvenile Cyprinodon variegatus that were wild caught in the Atlantic during 2014 | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Results from experiment examining effects of 4 different dyes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project) | | Aquarium |
Results from experiment examining effects of 2 different isotope spikes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project) | | Aquarium |
Physiological parameters of three corals species collected from Hawaii and exposed to four treatment conditions for 22-months as part of a mesocosm experiment | | mesocosm tank |
Wet and buoyant weight measurements of macroalgae at the Sitka Sound Science Center (SSSC) from August to September 2017 (High latitude kelp dynamics project) | To test the response of the coralline algae Crusticorallina spp. and Bossiella orbigniana to future OA scenarios, we used an 18-aquaria indoor experimental system with flow-through seawater at the Sitka Sound Science Center to simulate three static pHT levels (current summer = 8.0, future summer/current winter = 7.7, future winter = 7.4) under two seasonal light regimes simulated with full-spectrum aquarium lights (AI Prime HD) (summer = PPFD 55μmol m-2 s-1, 13h d-1, winter = PPFD 40μmol m-2 s-1, 6h d-1). We had a total of 3 aquaria for each of the 6 treatment combinations.
| 18-aquaria indoor experimental system |
Oxygen evolution data for macroalgae during photo physiology incubations for photosynthesis-irradiance curves from August to September 2017 (High latitude kelp dynamics) | To test the response of the coralline algae Crusticorallina spp. and Bossiella orbigniana to future OA scenarios, we used an 18-aquaria indoor experimental system with flow-through seawater at the Sitka Sound Science Center to simulate three static pHT levels (current summer = 8.0, future summer/current winter = 7.7, future winter = 7.4) under two seasonal light regimes simulated with full-spectrum aquarium lights (AI Prime HD) (summer = PPFD 55μmol m-2 s-1, 13h d-1, winter = PPFD 40μmol m-2 s-1, 6h d-1). We had a total of 3 aquaria for each of the 6 treatment combinations. A full description of the pH control for this system can be found in Kroeker et al. 2021, but in short: pH was regulated using a relay system that controlled mixing of pre-equilibrated low-pH seawater (formed by bubbling pure CO2 gas into seawater: pH6.0) and ambient pH seawater into 9 header buckets (n=3 headers per pH treatment) that then flowed into the experimental aquaria.
| 18-aquaria indoor experimental system with flow-through seawater at the Sitka Sound Science Center |
Environmental data from experimental aquaria during a laboratory experiment testing the effects of pH, light availability and biotic interaction on coralline algae calcification and productivity at the Sitka Sound Science Center in 2017 | To test the response of the coralline algae Crusticorallina spp. and Bossiella orbigniana to future OA scenarios, we used an 18-aquaria indoor experimental system with flow-through seawater at the Sitka Sound Science Center to simulate three static pHT levels (current summer = 8.0, future summer/current winter = 7.7, future winter = 7.4) under two seasonal light regimes simulated with full-spectrum aquarium lights (AI Prime HD) (summer = PPFD 55μmol m-2 s-1, 13h d-1, winter = PPFD 40μmol m-2 s-1, 6h d-1). We had a total of 3 aquaria for each of the 6 treatment combinations.
| 18-aquaria indoor experimental system with flow-through seawater at the Sitka Sound Science Center |
Acropora cervicornis coral disease exposure experiment conducted on Summerland Key, FL from August to September of 2015 (Resilient Acerv project) | One replicate of each genotype was placed within a single five gallon glass tank, holding approximately 10 liters of water. A total of ten tanks were used, five for the disease homogenate applications, and five for healthy homogenate applications.
Each tank, holding 10 L of water, were treated with either 100 ml of disease homogenate or 100 ml of healthy homogenate.
| Glass tanks |
Elemental compositions (Mg, Ca, Sr, N, C) of larval oyster shells from Whiskey Creek Shellfish Hatchery in Netarts Bay, OR, USA from 2009-2011 | | Tank |
Results from feeding assays conducted as part of seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species | | |
Feeding trials: Effects of diversity in feeding trials, conducted at Bodgea Marine Laboratory, using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers | Pre-weighed fragments of eelgrass were covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank.
| indoor tank |
Fertilization success rates from gamete age assays using eggs and sperm from Porites lobata corals in April-May 2023 | | flow-through seawater tanks |
Physiological metrics recorded at the end of experiments conducted to examine two coral species' responses to thermal stress | | |
Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress | | |
Field assay experiment data to determine if the presence of larger individuals of T. hardwicke adversely affects the survival of focal individuals in Moorea, French Polynesia from 2005-2007 (CDD_in_Reef_Fish project) | After tagging, fish were returned to aerated aquaria for 12 h before being measuring to the nearest 0.1 mm SL.
| aerated aquaria |
Data generated from the manipulative experiments in Moorea, French Polynesia from February-August 2007 (CDD_in_Reef_Fish project) | All captured fish were held in tanks with running seawater for 6–12 h, then individually tagged with different colors of Visible Implant Elastomer forward of the caudal peduncle.
| tanks |
Description of the background community of reefs used in an experiment at Moorea, French Polynesia from February-August 2007 (CDD_in_Reef_Fish project) | All captured fish were held in tanks with running seawater for 6–12 h, then individually tagged with different colors of Visible Implant Elastomer forward of the caudal peduncle.
| tanks |
Size of individuals used within each experiment at Moorea, French Poynesia from February-August 2007 (CDD_in_Reef_Fish project) | All captured fish were held in tanks with running seawater for 6–12 h, then individually tagged with different colors of Visible Implant Elastomer forward of the caudal peduncle.
| tanks |
Geochemistry and microscopy from incubation experiments of Chesapeake Bay sediments conducted at Horn Point Laboratory | | |
Cyprinodon variegatus offspring growth rate in grandparental experiments from specimens wild caught in the Atlantic during 2014 | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Experiment testing the temperature dependence of urchin grazing at the Galapagos Science Center on San Cristobal Island from February to March 2018 | Each mesocosm closed with a wire mesh top so that the water temperature inside the mesocosm was the same as that in the aquarium and water could be exchanged freely.
| aquarium |
Behavior Metrics - Crab-Oyster behavior - from UNC Reis laboratory starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | Crabs and oysters were raised in isolated 34 l tanks for 71 days in seawater
| Isolated 34 l tanks |
Consumption data from Crab-Oyster Behavior study from laboratory results from UNC from the Sapodilla Caye, Belize ongoing from 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | Crabs and oysters were raised in isolated 34 l tanks for 71 days in seawater
| Isolated 34 l tanks |
Seawater chemistry calculated parameters from Crab-Oyster Behavior study from laboratory results from UNC from data collected at the Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | Crabs and oysters were raised in isolated 34 l tanks for 71 days in seawater
| Isolated 34 l tanks |
Seawater chemistry measured parameters from Crab-Oyster Behavior study from laboratory results from UNC from data collected at the Sapodilla Caye, Belize starting 2010 ((OA - Ocean Acidification and Warming Impact on Calcification project) | Crabs and oysters were raised in isolated 34 l tanks for 71 days in seawater
| Isolated 34 l tanks |
Unsuccessful events from Crab-Oyster Behavior study from UNC laboratory results from data from the Sapodilla Caye, Belize starting 2010 (OA - Ocean Acidification and Warming Impact on Calcification project) | Crabs and oysters were raised in isolated 34 l tanks for 71 days in seawater
| Isolated 34 l tanks |
Invertebrate survival: Invertebrate survival rates from feeding experiments, conducted at Bodega Marine Laboratory, where food sources (eelgrass (Zostera marina) genotypes) were varied | Pre-weighed fragments of eelgrass were covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank.
| indoor tank |
Experimental results: calcification by primary coral polyps under high bicarbonate and low pH from 2007-2008 (OA Nutrition and Coral Calcification project) | Static, 30 L, glass-lidded aquaria containing reef seawater.
| Aquarium |
Kelp growth data from seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species | | |
Offspring growth rate from experiments testing for local adaptation in thermal TGP | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Net calcification of L. pertusa specimens exposed to different pH treatments collected on R/V Ronald Brown in Florida from October to November 2010 (Lophelia OA project) | 20 L aquaria were used on the ship and 570 L recirculating aquaria systems were used in the lab
| Aquarium |
Mortality of L. pertusa specimens exposed to different DO levels collected on R/V Ronald Brown in Florida from October to November 2010 (Lophelia OA project) | 20 L aquaria were used on the ship and 570 L recirculating aquaria systems were used in the lab
| Aquarium |
Mortality of L. pertusa specimens exposed to different temperatures collected on R/V Ronald Brown in Florida from October to November 2010 (Lophelia OA project) | 20 L aquaria were used on the ship and 570 L recirculating aquaria systems were used in the lab
| Aquarium |
Part 2 of a 2 part manipulative experiment to investigate the existence of cooperative synergy in defensive behaviors of ‘guard’ crustaceans at Gump Research Station, Moorea, French Polynesia from July 2006 (CDD_in_Reef_Fish project) | A coral colony with both Alpheus and Trapezia exosymbionts was placed on an elevated pedestal within a large seawater aquarium.
| aquarium |
Size of Bugula neritina colonies with and without symbiont grown at different temperatures in laboratory mesocosms at Georgia Tech | | tank |
Size of B. neritina colonies with and without symbiont grown at different temperatures | | tank |
Microbial taxa (amplicon sequence variant or ASV) statistical analyses for two seagrass genotypes from wasting disease mesocosm experiments at Bodega Marine Laboratory in July-Sept of 2015 | | flow through tanks |
Quantitative PCR cell count estimates from samples of DNA extracted from seagrass wasting disease parasite, Labyrinthula zosterae from wasting disease mesocosm experiments at Bodega Marine Laboratory in July-Sept of 2015 | | flow through tanks |
Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015 | | flow through tanks |
Temperature from seagrass wasting disease mesocosm experiments at Bodega Marine Laboratory in June-July 2015 | | flow through tanks |
Calcification and linear extension rates for Oculina arbuscula corals grown under different pCO2 levels; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project) | Specimens of O. arbuscula were reared in each of four 38-liter glass aquaria (76 specimens in total) filled with 0.2 um-filtered seawater.
| Aquarium |
Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015 | Coral colonies were fragmented into five replicate nubbins and placed into a 1200L flow-through aquarium and held at 27.5°C
| Flow-through aquarium |
Cyprinodon variegatus offspring growth rate from parental contribution experiments conducted on wild caught Atlantic specimens during 2014. | Used to acclimate juvenile sheepshead minnows
| Aquarium |
Photosynthesis and respiration data recorded during experiments conducted to examine two coral species' responses to thermal stress | | |
Physiological responses of the coral host and associated endosymbionts measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | |
Data from experiments testing the effects of hypoxia on behavior and physiology of two species of rockfish from from 2015-2016 | | |
Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | |
Nutrient transfer experiments with host coral and symbionts under varying environmental conditions conducted March 2014 and March 2015 | Colonies were fragmented into replicate pieces (clone ramets) and placed into 1200 L flow-through aquariums supplied with natural seawater and held at 27.5°C
| Flow through aquarium |
Results from mesocosm experiments measuring how temperature affects predation rates by whelks on barnacles | | Aquariums |