©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.An automated DNA size selection instrument, with pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. The instrument is used to automatically extract DNA fragments of a user selected size for downstream technologies such as miRNA isolation, DNA sequencing, RNA-seq, genotyping, DNA sequencing, ChIP-seq, and Long-read sequencing. The instrument uses electrophoresis along with laser detection or other imaging technology to determine when to start collecting DNA based on size ranges entered by the user. Once the DNA is no longer in the desired size range, collection ceases. The instrument has electrophoresis voltage options: 25V, 100V or 150V constant, or 100V pulsed field. The optical detection wavelength is 470 nm excitation, and 525 nm emission. The instrument can run up to 5 samples/gel cassettes at a time, with no possibility of cross contamination.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Coral associated microbes on coral, sediment, and water sampled from coral reefs in Mo'orea, French Polynesia from 2017 to 2020 | BluePippin is a an automated DNA Size Selection System, a preparative electrophoresis platform that uses agarose gel plates. For this dataset, DNA was size selected using Blue Pippin (Sage Scientific) prior to sequencing to minimize 12S sequence generation | BluePippin |
| Microbiome composition of coral species collected from reefs in Mo'orea, French Polynesia and exposed to various experimental treatments in August 2018 | ||
| 19 Metagenomes and 7 metatranscriptomes from sediment samples collected offshore of San Francisco, Califronia, USA | BluePippin |