©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
©2020 Biological and Chemical Oceanography Data Management Office.The AS-D1 is an instrument designed to prepare natural water samples for Dissolved Inorganic Carbon (DIC) and delta13C analysis and provide the user with the analyses outputs. It has features that are specifically useful for seawater and coastal water samples. The instrument provides the user with DIC values (micromol per kg) and the delta13C content of the DIC (per mille). It consists of a digital syringe pump for delivery of reagent and samples, a mass flow controller to regulate flow rate, a CO2 stripping reactor, and an electronic cooling system to remove moisture. The AS-D1 does not measure the sample but is designed to send the gas to a different analyzer. This second instrument then sends the measurements back to the AS-D1 after analysis. The AS-D1 then calculates the desired DIC and delta13C outputs. This instrument is designed for automatic sampling from multiple bottles. It can be used in laboratories on shore or at sea.
The instrument was created to be paired with the Picarro G-2131i Carbon Isotope Analyser, however, other models that measure the isotopic ratio of CO2 may be compatible. The precision is +/- 0.1 % for DIC of seawater and +/- 0.07 % for DIC-delta13C. Sample volume is 1-7 milliliters per analysis, and sample time is under 12 minutes.
Additional information from the manufacturer is available at: https://apolloscitech.com/dicdelta.html
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Hatching count and survival statistics of Atlantic silverside (Menida menidia) larvae reared under ambient or hypoxia/acidification treatments during cross-generational laboratory experiments in 2022 | Samples were measured in triplicate for dissolved inorganic carbon (DIC) using an Apollo AS-D1 analyzer connected to a Picarro G-2121i cavity ringdown system. | Apollo AS-D1 analyzer connected to a Picarro G-2121i cavity ringdown system |
| Morphological measurements of Atlantic silverside (Menidia menidia) larvae reared under ambient or hypoxia/acidification treatments during cross-generational laboratory experiments in 2022 | Samples were measured in triplicate for dissolved inorganic carbon (DIC) using an Apollo AS-D1 analyzer connected to a Picarro G-2121i cavity ringdown system. | Apollo AS-D1 analyzer connected to a Picarro G-2121i cavity ringdown system |
| Bottle data from R/V Endeavor cruise EN665 in the Gulf of Maine, conducted April 7-12, 2021 | DIC-d13C was determined using an Apollo AS-D1 DIC and d13C-DIC analyzer unit, connected to a Picarro G-2121i Cavity Ringdown Spectrometer. | Apollo AS-D1 DIC and d13C-DIC analyzer unit |
| Carbonate chemistry analyses (total alkalinity, DIC, and d13C of DIC) from discrete bottle samples and CTD data from 12 stations sampled during R/V Endeavor cruise EN669 in the Gulf of Maine during August 2021 | DIC-d13C was determined using an Apollo AS-D1 DIC and d13C-DIC analyzer unit, connected to a Picarro G-2121i Cavity Ringdown Spectrometer. | Apollo AS-D1 analyzer |
| A G2131-i Isotope and Gas Concentration CRDS Analyzer (Picarro, USA) was employed along with an AS-D1 δ13C-DIC Analyzer (Apollo SciTech, USA) for sample injection, CO2 extraction, instrument control, and data acquisition in δ13C-DIC measurements. | AS-D1 δ13C-DIC Analyzer (Apollo SciTech, USA) |