Dataset: Seawater carbonate chemistry from experiment on brooded coral larval, March 2011, Taiwan (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)

Final no updates expectedVersion 1 (2014-10-07)Dataset Type:experimental

Principal Investigator: Peter J. Edmunds (California State University Northridge)

Co-Principal Investigator: Dr Vivian R Cumbo (California State University Northridge)

Co-Principal Investigator: Tung-Yung Fan (National Museum of Marine Biology and Aquarium)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Long Term Ecological Research network (LTER)

Project: Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

Project: The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)


Abstract

The physiological development of brooded larvae from the pocilloporid corals Pocillopora damicornis in southern Taiwan under elevated temperature and pCO2 was examined. These data include seawater carbonate chemistry from experiments on the physiological development of brooded coral larvae conducted in March 2011. Reported parameters include temperature, salinity, pH, total alkalinity, pCO2, bicarbonate and carbonate concentration, and the saturation state (omega) for aragonite. These data were ...

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Treatments were created in eight 150 L tanks, each filled with 120 L of filtered (1 um) seawater that was changed partially (20%) every day (at ~17:00 h). Tanks were individually heated (300 Wheaters, Taikong Corporation) and chilled (Aquatech Ac11 or Shyeh Duwai Enterprise), with the temperature regulated using programmable, digital controllers (±0.1 °C, AquaControllers, Neptune Systems). Illumination was provided by metal halide (Phillips 150 W 10,000 k) and fluorescent (39 W, Phillips T5 460 nm) bulbs to create a mean light intensity of 268± 17 µmol quanta m-2 s-1 (±SE, n=64). The light intensity was selected to approximate that found at the collection depth of the parent colonies in March.

Treatments were created by blending CO2 with air, and continually assessing the mixture through an Infra Red gas analyzer (S151, Qubit Systems), which dynamically adjusted the flow of CO2 to maintain desired levels. The gas mixture was supplied through an air stone to four of the eight tanks, with others supplied with compressed air (i.e., ambient pCO2). The conditions in the tanks were analyzed for pH, salinity, temperature, and total alkalinity (TA) using standard operating procedures (Dickson et al., 2007), and the program CO2SYS (Lewis and Wallace, 1998) to calculate DIC parameters. The gas mixing technology and the methods for seawater analyses essentially are identical to those we have used before (see Dufault et al., 2012; Edmunds, 2011). In the present analysis, the calculated TA values of certified reference materials supplied by Dr. Andrew Dickson, Scripps Institute of Oceanography (batch no. 98 and 107), were determined within a mean of 1% of the certified value.

The incubation system created target temperatures of ~24.00 °C versus 30.50 °C, and target pCO2 values of ambient versus 86.1 Pa, and an irradiance of ~268 µmol quanta m-2 s-1. Conditions in the tanks were measured at least daily using a certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C), a cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400), and a sample ofwater withdrawn from each tank for pH, salinity, and TA analysis.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

​Data also available from PANGAEA: doi:10.1594/PANGAEA.823582


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Dataset: brooded coral larvae 3 - light
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Light data in tanks from experiment on brooded coral larval, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535219
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Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535425
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Related Publications

Results

Cumbo, V. R., Fan, T. Y., & Edmunds, P. J. (2013). Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. Journal of Experimental Marine Biology and Ecology, 439, 100–107. doi:10.1016/j.jembe.2012.10.019
Results

Dufault, A. M., Cumbo, V. R., Fan, T.-Y., & Edmunds, P. J. (2012). Effects of diurnally oscillating pCO2 on the calcification and survival of coral recruits. Proceedings of the Royal Society B: Biological Sciences, 279(1740), 2951–2958. doi:10.1098/rspb.2011.2545
Methods

Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication 3, 191 pp
Methods

Edmunds, P. J. (2011). Zooplanktivory ameliorates the effects of ocean acidification on the reef coral Porites spp. Limnology and Oceanography, 56(6), 2402–2410. doi:10.4319/lo.2011.56.6.2402
Methods

Pierrot, D. E. Lewis,and D. W. R. Wallace. 2006. MS Excel Program Developed for CO2 System Calculations. ORNL/CDIAC-105a. Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, Tennessee. doi: 10.3334/CDIAC/otg.CO2SYS_XLS_CDIAC105a.