Dataset: Respiration of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)

Final no updates expectedVersion 1 (2014-10-07)Dataset Type:experimental

Principal Investigator: Peter J. Edmunds (California State University Northridge)

Co-Principal Investigator: Dr Vivian R Cumbo (California State University Northridge)

Co-Principal Investigator: Tung-Yung Fan (National Museum of Marine Biology and Aquarium)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Program: Long Term Ecological Research network (LTER)

Project: Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

Project: The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)


Abstract

The physiological development of brooded larvae from the pocilloporid corals Pocillopora damicornis in southern Taiwan under elevated temperature and pCO2 was examined. These data include respiration rates of brooded coral larvae at high and ambient temperature and pCO2 conducted in March 2011. These data were published in Cumbo et al, JEMBE, 2013.

Larval respiration was measured in a confined respirometer use a Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation usingwater-saturated air at the treatment temperature. Tomeasure respiration, 6 larvae were removed from the tubs in the treatment tanks and placed into 2-mL glass Wheaton vials filled with filtered seawater from the same treatment tank and sealed with Parafilm. A study conducted concurrently with the present analysis demonstrated that respiration of P. damicornis larvae in identical vials could be measured accurately with 5 larvae vial-1 (Edmunds et al., 2011).

Initial O2 concentration in the seawater was determined before the vials were sealed, and vials without larvae used as controls. Larvae in the sealed vials were incubated at their temperature treatments for 1.5-2 h in the dark using water baths set to the temperature of the respective treatments (±0.1 °C, Hipoint, models LC-06 and LC-10); vials were carefully moved throughout the incubations to ensure the seawater remained mixed. Incubation timeswere selected to ensure O2 concentrations remained >75% saturation. On completion of the incubations, vials were removed from the water baths, gently inverted to mix the seawater, and analyzed for O2 saturation. O2 saturation was converted to concentration using tabulated values of O2 solubility (N. Ramsing and J. Gundersen at http://www.unisense.com [based on Garcia and Gordon, 1992]) and the temperature and salinity of the seawater, and the change in O2 concentration used to calculate O2 flux after adjusting for controls. The respiration rates are expressed as nmol O2 mg protein-1 min-1.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582


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Dataset: brooded coral larvae 3 - light
Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Light data in tanks from experiment on brooded coral larval, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535219
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Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535425
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Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Respiration and protein content of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535328
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Edmunds, P. J., Cumbo, V. R., Fan, T. (2014) Seawater carbonate chemistry from experiment on brooded coral larval, March 2011, Taiwan (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2014-10-07 http://lod.bco-dmo.org/id/dataset/535163
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Related Publications

Results

Cumbo, V. R., Fan, T. Y., & Edmunds, P. J. (2013). Effects of exposure duration on the response of Pocillopora damicornis larvae to elevated temperature and high pCO2. Journal of Experimental Marine Biology and Ecology, 439, 100–107. doi:10.1016/j.jembe.2012.10.019
Results

Edmunds, P. J., Cumbo, V., & Fan, T.-Y. (2011). Effects of temperature on the respiration of brooded larvae from tropical reef corals. Journal of Experimental Biology, 214(16), 2783–2790. doi:10.1242/jeb.055343
General

Garcia, H. E., & Gordon, L. I. (1992). Oxygen solubility in seawater: Better fitting equations. Limnology and Oceanography, 37(6), 1307–1312. doi:10.4319/lo.1992.37.6.1307