Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.
Shimadzu 10AD HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 µm) reverse-phase C18 column in series.
Mass spectrometry samples were analyzed on a Vanquish UHPLC system (Thermo Fisher Scientific) coupled via heated electrospray ionization (H-ESI) to an ultrahigh resolution tribrid mass spectrometer, the Orbitrap Fusion Lumos (Thermo Fisher Scientific).
Ultrahigh performance liquid chromatography system (Vanquish UHPLC, Thermo ScientificTM) coupled with an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo ScientificTM) for untargeted metabolomics
ACQUITY UPLC system (Waters) equipped with a photodiode array detector and a C8 column (1.7 μm particle size, 2.1 mm internal diameter, 50 mm column length, ACQUITY UPLC BEH, 186002877)
Targeted metabolomics were measured using the ultra-performance liquid chromatography (Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific) coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM) mode at the Woods Hole Oceanographic Institution (WHOI).
Accela Open Autosampler and Accela 1250 Pump, Thermo Scientific)