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Dataset: FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018

Final no updates expectedDOI: 10.26008/1912/bco-dmo.887787.1Version 1 (2023-01-27)Dataset Type:Cruise Results

William M. Balch (Principal Investigator)

Stephen D. Archer (Co-Principal Investigator)

David T. Drapeau (Co-Principal Investigator)

Jelena Godrijan (Co-Principal Investigator)

Dana Stuart Gerlach (BCO-DMO Data Manager)

Project: Coccolithophore Mixotrophy (Cocco-Mix)

Abstract

This dataset presents imaging cytometer data from water samples collected during R/V Endeavor cruise EN616. Niskin bottle samples were taken at nine stations and eight depths in the northwest Atlantic in July 2018. A Yokogawa FlowCAM imaging cytometer was used to enumerate the major microalgal classes, and the particle size distribution function was estimated.

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This dataset is part of a larger study with the following goals:

  • Goal #1: measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and BCO-DMO dataset: https://www.bco-dmo.org/dataset/858771). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy of coccolithophores from that by other naturally-occurring microalgae.
  • Goal #2: test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.

Related Datasets

IsRelatedTo
Balch, W. M., Godrijan, J., Drapeau, D. T., Archer, S. D. (2023) DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-22 doi:10.26008/1912/bco-dmo.887562.1
[view at BCO-DMO]
IsRelatedTo
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-26 doi:10.26008/1912/bco-dmo.887851.1
[view at BCO-DMO]
IsSupplementedBy
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Hydrography and environmental conditions measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.887800.1
[view at BCO-DMO]
IsRelatedTo
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 doi:10.26008/1912/bco-dmo.887863.1
[view at BCO-DMO]
IsRelatedTo
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 doi:10.26008/1912/bco-dmo.887863.1
[view at BCO-DMO]

Related Publications

Results
Balch, W. M., Drapeau, D. T., Poulton, N., Archer, S. D., Cartisano, C., Burnell, C., & Godrijan, J. (2023). Osmotrophy of dissolved organic compounds by coccolithophore populations: Fixation into particulate organic and inorganic carbon. Science Advances, 9(21). https://doi.org/10.1126/sciadv.adf6973
Methods
Menden-Deuer, S., & Lessard, E. J. (2000). Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography, 45(3), 569–579. doi:10.4319/lo.2000.45.3.0569
Methods
Poulton, N. J. and Martin, J.L. (2010). Imaging flow cytometry for quantitative phytoplankton analysis — FlowCAM. In: Intergovernmental Oceanographic Commission of ©UNESCO. Karlson, B., Cusack, C. and Bresnan, E. (editors). Microscopic and molecular methods for quantitative phytoplankton analysis. Paris, UNESCO. (IOC Manuals and Guides, no. 55.) (IOC/2010/MG/55), 110 pages. Available from: https://unesdoc.unesco.org/ark:/48223/pf0000187824

Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.887787.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation