Dataset: Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018

Final no updates expectedDOI: 10.26008/1912/bco-dmo.887863.1Version 1 (2023-02-05)Dataset Type:Cruise Results

Principal Investigator: William M. Balch (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Stephen D. Archer (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: David T. Drapeau (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Jelena Godrijan (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

Project: Coccolithophore Mixotrophy (Cocco-Mix)

Abstract

This dataset presents polarized microscopy-derived concentration data for coccolithophores and detached coccoliths in samples collected from stations in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Counts are based on image analysis of dark-field, cross-polarized views of filtered particulate matter. These counts take advantage of the birefringence property of calcium carbonate (particulate inorganic carbon) that it rotates the plane of linearly polarized incident light ...

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Cite Dataset

This dataset is part of a larger study with the following goals:

  •  Goal #1: measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al., 2020). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy of coccolithophores from that by other naturally-occurring microalgae.
  • Goal #2: test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.

Related Datasets

IsRelatedTo
Dataset: Data for ambient concentrations of three DOC compounds (acetate, glycerol, mannitol)
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-26 doi:10.26008/1912/bco-dmo.887851.1
IsRelatedTo
Dataset: FlowCAM imaging cytometer data from EN616 cruise
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27 doi:10.26008/1912/bco-dmo.887787.1
IsRelatedTo
Dataset: EN616 CTD hydrography
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Hydrography and environmental conditions measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.887800.1
IsRelatedTo
Dataset: EN616 Coccolithophore DOC uptake and scintillation counts
Balch, W. M., Godrijan, J., Drapeau, D. T., Archer, S. D. (2023) DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-22 doi:10.26008/1912/bco-dmo.887562.1

Related Publications

Results
Balch, W. M., Drapeau, D. T., Poulton, N., Archer, S. D., Cartisano, C., Burnell, C., & Godrijan, J. (2023). Osmotrophy of dissolved organic compounds by coccolithophore populations: Fixation into particulate organic and inorganic carbon. Science Advances, 9(21). https://doi.org/10.1126/sciadv.adf6973
Methods
Balch, W., & Utgoff, P. (2009). Potential Interactions Among Ocean Acidification, Coccolithophores, and the Optical Properties of Seawater. Oceanography, 22(4), 146–159. https://doi.org/10.5670/oceanog.2009.104
Related Research
Godrijan, J., Drapeau, D., & Balch, W. M. (2020). Mixotrophic uptake of organic compounds by coccolithophores. Limnology and Oceanography, 65(6), 1410–1421. doi:10.1002/lno.11396

Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.887863.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation