Dataset: Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018

Final no updates expectedDOI: 10.26008/1912/bco-dmo.887851.1Version 1 (2023-02-26)Dataset Type:Cruise Results

Principal Investigator: William M. Balch (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Stephen D. Archer (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: David T. Drapeau (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Jelena Godrijan (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

Project: Coccolithophore Mixotrophy (Cocco-Mix)

Abstract

This data set provides ambient concentrations of three dissolved organic compound (acetate, glycerol and mannitol) measured from water samples taken during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. These concentrations were derived using new analytical methods described in the below-referenced Science Advances manuscript by Balch et al.

Cite Dataset

This dataset is part of a larger study with the following goals:

  •  Goal #1: measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and BCO-DMO dataset: https://www.bco-dmo.org/dataset/858771). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy of coccolithophores from that by other naturally-occurring microalgae.
  • Goal #2: test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.

Related Datasets

IsRelatedTo
Dataset: Coccolithophore birefringence from polarized microscopy
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 doi:10.26008/1912/bco-dmo.887863.1
IsRelatedTo
Dataset: FlowCAM imaging cytometer data from EN616 cruise
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27 doi:10.26008/1912/bco-dmo.887787.1
IsSupplementTo
Dataset: EN616 Coccolithophore DOC uptake and scintillation counts
Balch, W. M., Godrijan, J., Drapeau, D. T., Archer, S. D. (2023) DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-22 doi:10.26008/1912/bco-dmo.887562.1
IsSupplementedBy
Dataset: EN616 CTD hydrography
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Hydrography and environmental conditions measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.887800.1

Related Publications

Results
Balch, W. M., Drapeau, D. T., Poulton, N., Archer, S. D., Cartisano, C., Burnell, C., & Godrijan, J. (2023). Osmotrophy of dissolved organic compounds by coccolithophore populations: Fixation into particulate organic and inorganic carbon. Science Advances, 9(21). https://doi.org/10.1126/sciadv.adf6973
Methods
Garland, P. B., & Randle, P. J. (1962). A Rapid Enzymatic Assay for Glycerol. Nature, 196(4858), 987–988. https://doi.org/10.1038/196987a0
Methods
King, G. M. (1991). Measurement of Acetate Concentrations in Marine Pore Waters by Using an Enzymatic Approach. Applied and Environmental Microbiology, 57(12), 3476–3481. https://doi.org/10.1128/aem.57.12.3476-3481.1991
Methods
Kiyoshima, A., Kudo, K., Hino, Y., & Ikeda, N. (2001). Sensitive and simple determination of mannitol in human brain tissues by gas chromatography–mass spectrometry. Journal of Chromatography B: Biomedical Sciences and Applications, 758(1), 103–108. https://doi.org/10.1016/s0378-4347(01)00145-1
Related Research
Godrijan, J., Drapeau, D. T., & Balch, W. M. (2021). Osmotrophy of dissolved organic carbon by coccolithophores in darkness. New Phytologist, 233(2), 781–794. doi:10.1111/nph.17819

Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.887851.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation