Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
Dataset Name | Brief Description |
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2_feeding-copepod chlorophyll uptake | Chlorophyll uptake by adult and nauplius copepod species on three phytoplankton species |
3a_gut evacuation time | Experiment to test copepod gut evacuation time |
3b_minimum readable gut fluorescence | Experiment to find minimum time for copepod gut fluorescence by plate reader method |
3c_copepod sonication for chl extraction | Experiment to test whether sonication is needed for chlorophyll extraction from copepods |
4b_min fluorescence thresholds | Pseudodiaptomus marinus gut minimum fluorescence thresholds |
AE1812 Alkaline phosphatase | Alkaline phosphatase activities |
Anderson & Rynearson 2020: Skeletonema thermal growth | Anderson & Rynearson 2020: Skeletonema thermal growth |
APA Bioassay Experiments | |
APA In-situ Experiments | |
Atlantic silverside metabolic rates | CO2, temperature, and oxygen effects on Menidia menidia metabolic rates |
Biochemistry of sponges | |
BioLog Eco-plates experiment - Metabolic potential | BioLog Eco-plates experiment - Metabolic potential |
brooded coral larvae 1 - size_protein_symbionts_photosynth | Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012) |
brooded coral larvae 3 - protein | Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) |
brooded coral larvae 3 - respiration and protein | Respiration and protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) |
Carbonate chemistry | |
Chemotaxis of P. haloplanktis towards exudates of Synechoccocus | |
Chemotaxis of V. alginolyticus towards Synechococcus Cells | Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells |
Coral Colony Physiological Summary | |
Coral Colony Physiological Summary | |
coral microbe T-RF - temp stress | Terminal restriction fragments from 16S rRNA genes amplified from coral-associated microbes, response to temperature stress |
coral-microbe T-RF - development | Specificity of associations between bacteria and the coral Pocillopora meandrina during early development |
coral-microbe T-RF - spawning | T-RFLP, response of the microbial community to coral spawning, lagoon and reef flats, Oahu, 2006-7 |
Effects of protists on HBx predation | Population Changes |
Emiliania size and chemical characteristics | Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi. |
EN556 bulk peptidase hydrolysis rates - plate reader | Bulk polysaccharide hydrolysis rates - EN556 |
EN556 gravity filtered polysaccharide/glucosidase hydrolysis rates | Gravity filtered polysaccharide hydrolysis rates - EN556 |
EN584 LV incubation, peptidase and glucosidase activities | Large volume incubation, peptidase and glucosidase activities (plate reader), EN584 |
EN638 Bulk Plate Rates | |
EN638 LV Plate Rates | Substrate hydrolysis |
Field collections: all data | Vibrio abundance and nutrients |
Fig2_copepod feeding on prey mixtures | Copepod fed phytoplankton mixtures |
Final Physiology | Final Physiology |
Fv/Fm | Fv/Fm |
Heatwave Experiment: Multivariate Physiology | Heatwave Experiment: Multivariate Physiology |
High resolution screen growth curves | |
High Throughput Growth Screen Data | High Throughput Growth Screening Ruegeria pomeroyi |
Host-Symbiont respiration relationship | Host-symbiont respiration related to symbiont density; anemones from Key Largo |
Hydrolytic enzyme activity - Coscinodiscus | Bacterial cell counts during CDOM Coscinodiscus monoculture experiment |
Hydrolytic enzyme activity - other phytoplankton | Bacterial cell counts during CDOM monoculture experiment |
IODP360 - Replicate Cell Counts | |
Montipora antibacterial-aqueous | Antibacterial assays of Montipora capitata aqueous extracts |
Montipora antibacterial-mucus | Antibacterial assays of Montipora capitata mucus |
OC1703A and AT36 sediment geochemistry | OC1703A and AT36 sediment pore water geochemistry |
Phosphohydrolysis rates in the coastal western North Atlantic | Phosphohydrolysis rates in the coastal western North Atlantic |
Photosynthesis and Respiration | Photosynthesis and Respiration |
Physiological Response Metrics | Physiological Response Metrics |
Phytoplankton phosphonate utilization | |
Polyphosphate in the Great Lakes | Polyphosphate in the Great Lakes (USA) |
Primary producer amino acid carbon isotopes | Primary producer amino acid carbon isotopes |
Prochlorococcus and Synechococcus nitrite accumulation in batch culture | Prochlorococcus and Synechococcus nitrite accumulation in batch culture |
Quantification of ciliated band length per unit protein in early echinoderm larvae: protein data | Protein content of early echinoderm larvae |
RB-TnSeq Data | |
Red Sea Gradient Physiology | Red Sea Gradient Physiology |
RNA:DNA Ratios and Total lipids – experiment data | |
Ruegeria pomeroyi DOP hydrolysis rates | |
Seasonal iron biogeochemistry | Pore water and solid phase iron geochemical data |
Seeded microcosm experiments: all data | Nutrients from seeded microcosm experiments |
Single amplified genomes | Single amplified genomes (SAGs) |
Specific Dynamic Action of larval grunion - Experiment 1 | Specific Dynamic Action of larval grunion - Experiment 1 |
Specific Dynamic Action of larval grunion - Experiments 2a and 2b | Specific Dynamic Action of larval grunion - Experiments 2a and 2b |
Syn-Pro Co-cultures | Syn-Pro Co-cultures |
Synechococcus DOP Displacement Experiment | |
Synechococcus DOP Hydrolysis Experiment - cell counts and IVF | |
Synechococcus DOP Hydrolysis Experiment - hydrolysis rates | |
Synechococcus fluorescence emission spectra | Synechococcus fluorescence emission spectra |
Temperature | Temperature |
Tissue data from Keister et al. 2023 | Palau coral comparative analysis thermal tolerance physiology |
TPC Growth rates: 100 generations | growth at 7 temps after 100 generations |
TPC Growth rates: 200 generations | TPC growth at 8 temps after 200 generations |
Water_Column_AE1409 | Water column data from cruise AE1409 |
Zooplankton metabolic traits | Respirometry data for pelagic crustaceans, cephalopods, and fish |